Song Yonghong, Silversides Fred G
Agassiz Research Centre, Agriculture and Agri-Food Canada, Agassiz, BC, Canada.
Anim Reprod Sci. 2008 May;105(3-4):430-7. doi: 10.1016/j.anireprosci.2007.12.024. Epub 2008 Jan 9.
This study aimed to extend techniques of ovarian transplantation from chickens to Japanese quail. Ovarian tissue was surgically transplanted from chicks obtained from a line of white-breasted (wb/wb) to those of wild-type (+/+) quail at 1-day of age or at 1 week, with or without subsequent administration of the immunosuppressant, mycophenolate mofetil. Only one out of seven quail (14.3%) transplanted at 1-day of age survived the surgery but 14 of 18 quail (77.8%) transplanted at a week of age survived and grew to sexual maturity. In the 8-week progeny test, among those quail that produced offspring, one of four in the non-immunosuppressed group and all five in the immuosuppressed group produced donor-derived offspring. The proportion of donor-derived to host-derived offspring from five quail in the immnuosuppressed group (173/57) was significantly higher than that from one quail that was not immunosuppressed (3/47). These results suggest that quail ovaries can be transplanted at the age of 1 week and that the immnunosuppressant, mycophenolate mofetil, used for mammalian organ transplantation can suppress immunological rejection in birds. These transplantation techniques could provide an efficient means of recovering a line of quail after cryopreservation.
本研究旨在将卵巢移植技术从鸡扩展到日本鹌鹑。在1日龄或1周龄时，将来自白胸（wb/wb）品系雏鸡的卵巢组织手术移植到野生型（+/+）鹌鹑体内，移植后有的给予免疫抑制剂霉酚酸酯，有的未给予。1日龄移植的7只鹌鹑中只有1只（14.3%）在手术后存活，但1周龄移植的18只鹌鹑中有14只（77.8%）存活并发育到性成熟。在8周的子代测试中，在那些产生后代的鹌鹑中，未免疫抑制组的4只中有1只，免疫抑制组的5只全部产生了供体来源的后代。免疫抑制组5只鹌鹑的供体来源后代与宿主来源后代的比例（173/57）显著高于未免疫抑制的1只鹌鹑（3/47）。这些结果表明，鹌鹑卵巢可在1周龄时进行移植，并且用于哺乳动物器官移植的免疫抑制剂霉酚酸酯可抑制鸟类的免疫排斥反应。这些移植技术可为冷冻保存后鹌鹑品系的恢复提供一种有效的方法。
