Djukovic Danijel, Appiah-Amponsah Emmanuel, Shanaiah Narasimhamurthy, Gowda G A Nagana, Henry Ian, Everly Mike, Tobias Brian, Raftery Daniel
Department of Chemistry, Purdue University, West Lafayette, IN 47907, USA.
J Pharm Biomed Anal. 2008 Jun 9;47(2):328-34. doi: 10.1016/j.jpba.2007.12.035. Epub 2007 Dec 28.
Solid-phase extraction and column-trapping preconcentration are combined to enhance HPLC-nuclear magnetic resonance (HPLC-NMR) and applied to metabolite profiling in biological samples. Combining the two signal enhancement techniques improved the NMR signal substantially such that we were able to identify 2-hydroxyibuprofen, carboxyibuprofen, and unmetabolized ibuprofen molecules from a small urine sample after a therapeutic dose of ibuprofen. The hyphenated SPE/column-trapping method resulted in an excellent overall signal enhancement of up to 90-fold.
将固相萃取和柱捕集预浓缩相结合，以增强高效液相色谱 - 核磁共振联用技术（HPLC-NMR），并应用于生物样品中的代谢物谱分析。将这两种信号增强技术相结合，显著提高了核磁共振信号，使得我们能够在给予治疗剂量的布洛芬后，从小份尿液样本中鉴定出2-羟基布洛芬、羧基布洛芬和未代谢的布洛芬分子。联用的固相萃取/柱捕集方法实现了高达90倍的出色总体信号增强。
