Hattori Kimihiro, Matsui Hiroki
Graduate School of Bioresources, Mie University, Tsu, Mie, Japan.
Anaerobe. 2008 Apr;14(2):87-93. doi: 10.1016/j.anaerobe.2007.12.002. Epub 2007 Dec 28.
Diversity of fumarate reducing (dissimilating) bacteria in the bovine rumen was analyzed by both culture dependent and independent methodologies. A total of 39 strains were isolated by using three different media and belonged to three different phyla (Proteobacteria, Fusobacteria, and Firmicutes). A primer set that amplified the fumarate reductase gene (frdA) from Proteobacteria was developed and two frdA clone libraries were constructed. Identities of deduced amino acid sequences of cloned frdA amplicons against known sequences ranged from 58% to 85% suggesting the presence of unknown fumarate reducing bacteria. This is the first report on the diversity of fumarate reducing bacteria in the rumen.
采用依赖培养和不依赖培养的方法分析了牛瘤胃中延胡索酸还原（异化）细菌的多样性。使用三种不同培养基共分离出39株菌株，它们分属于三个不同的门（变形菌门、梭杆菌门和厚壁菌门）。开发了一组从变形菌门中扩增延胡索酸还原酶基因（frdA）的引物，并构建了两个frdA克隆文库。克隆的frdA扩增子推导氨基酸序列与已知序列的同一性范围为58%至85%，这表明存在未知的延胡索酸还原细菌。这是关于瘤胃中延胡索酸还原细菌多样性的首次报道。
