Lacinová Z, Michalský D, Kasalický M, Dolinková M, Haluzíková D, Roubícek T, Krajícková J, Mráz M, Matoulek M, Haluzík M
III. interní klinika 1. lékarské fakulty UK a VFN Praha.
Vnitr Lek. 2007 Nov;53(11):1190-7.
The objective of the study was to measure the concentration of adiponectin in plasma, its mRNA expression and the expression of the adiponectin receptors AdipoR1 and AdipoR2 in subcutaneous adpipose tissue (ST) of women with various levels of fat in their organism. A further objective of the study was to determine to what extent the stated parameters correlate with obesity as defined by BMI (body mass index) and how it can be affected by a very low calorie diet (VLCD).
The sample of 70 women was divided into groups by BMI: patients with class 3 obesity (BMI > 40 kg.m(-2), n = 25), patients with class 1 and 2 obesity (BMI 30-40 kg.m(-2), n = 15), overweight patients (BMI 25-30 kg.m(-2), n = 10) and a normal healthy control group (BMI 20-25 kg.m(-2), n = 20). In the case of 14 women with class 3 obesity, the parameters were measured before and after a three-week diet with an energy content of 2200 kJ (550 kcal)/day (VLCD).
Plasma concentrations of adiponectin were measured using an ELISA kit (LINCO Research, USA). Subcutaneous adipose tissue was taken using biopsy. RNA was isolated using a MagNA Pure Compact RNA Isolation Kit (Tissue) (Roche, SRN). The gene expression of adiponectin, AdipoR1 and AdipoR2 was determined using the real-time PCR (RT-PCR) method on a ABI Real-Time PCR 7500 instrument (Applied Biosystems, USA) with TaqMan Gene Expression Assays hydrolisation probes. beta-2-mikroglobulin (beta2M) was used as an endogenous control, to which the data was normalised.
The circulatory concentration of adiponectin, its mRNA expression and the mRNA expression of AdipoR1 in ST correlate negatively with BMI (r = -0.524, p < 0.001; r = -0.460, p < 0 001; p = -0.354, p = 0.004). The expression of AdipoR2 in ST did not correlate with BMI. The VLCD reduced weight by 9% but did not affect the plasma concentration of adiponectin or the rate of its expression, or the expression of AdipoR1 and AdipoR2.
Our results show that not only the circulatory concentration of adiponectin but also its mRNA expression and the expression of AdipoR1 in ST are significantly lower in obese women compared to a control group and may contribute to the development of metabolic complications in obesity.
本研究的目的是测量不同体脂水平女性血浆中脂联素的浓度、其mRNA表达以及皮下脂肪组织（ST）中脂联素受体AdipoR1和AdipoR2的表达。本研究的另一个目的是确定上述参数与BMI（体重指数）定义的肥胖之间的相关程度，以及极低热量饮食（VLCD）如何影响这些参数。
70名女性样本根据BMI分为几组：3级肥胖患者（BMI>40kg·m⁻²，n = 25）、1级和2级肥胖患者（BMI 30 - 40kg·m⁻²，n = 15）、超重患者（BMI 25 - 30kg·m⁻²，n = 10）和正常健康对照组（BMI 20 - 25kg·m⁻²，n = 20）。对于14名3级肥胖女性，在进行为期三周、能量含量为2200kJ（550kcal）/天的饮食（VLCD）前后测量各项参数。
使用ELISA试剂盒（美国LINCO Research公司）测量血浆中脂联素的浓度。通过活检获取皮下脂肪组织。使用MagNA Pure Compact RNA分离试剂盒（组织）（罗氏公司，SRN）分离RNA。使用ABI实时PCR 7500仪器（美国应用生物系统公司）上的TaqMan基因表达分析水解探针，通过实时PCR（RT-PCR）方法测定脂联素、AdipoR1和AdipoR2的基因表达。β-2-微球蛋白（β2M）用作内参，数据以此进行归一化。
脂联素的循环浓度、其mRNA表达以及ST中AdipoR1的mRNA表达与BMI呈负相关（r = -0.524，p < 0.001；r = -0.460，p < 0.001；r = -0.354，p = 0.004）。ST中AdipoR2的表达与BMI无相关性。VLCD使体重减轻了9%，但未影响血浆中脂联素的浓度、其表达速率或AdipoR1和AdipoR2的表达。
我们的结果表明，与对照组相比，肥胖女性不仅血浆中脂联素的浓度，而且其mRNA表达以及ST中AdipoR1的表达均显著降低，这可能导致肥胖患者发生代谢并发症。
