[爱泼斯坦-巴尔病毒潜伏膜蛋白1上调埃兹蛋白表达对鼻咽癌细胞转移能力的影响]

[Impact of up-regulating Ezrin expression by Epstein-Barr virus latent membrane protein 1 on metastasis ability of nasopharyngeal carcinoma cells].

作者信息

Shen Zhi-Hua, Chen Xiao-Yi, Chen Jin

机构信息

Department of Pathophysiology, Guangdong Medical College, Zhanjiang, Guangdong, 524023, P. R. China.

出版信息

Ai Zheng. 2008 Feb;27(2):165-9.

PMID:18279614

Abstract

BACKGROUND & OBJECTIVE: Ezrin, a cytoskeleton-related protein, contributes to the invasion and metastasis of tumors; our previous study had also shown that Epstein-Barr virus latent membrane protein 1 (EBV-LMP1) can promote the metastasis of nasopharyngeal carcinoma (NPC) cells. This study was to investigate whether EBV-LMP1 could change the expression of Ezrin and the role of Ezrin on the metastasis of NPC cells.

METHODS

The expression of EBV-LMP1 and Ezrin in NPC cell lines CNE1 (well differentiated NPC cell line) and CNE1-GL (CNE1 cells stably transfected with EBV-LMP1) were detected by SP immunohistochemistry and Western blot. The adhesive abilities of CNE1, CNE1-GL and AntiEzrin-CNE1-GL (CNE1-GL cells pretreated with Ezrin antibody) cells were assessed by cell-matrix adhesion assay. The invasion and migration of these cell lines were investigated by Transwell method.

RESULTS

Ezrin was not expressed in CNE1 cells while strongly expressed in CNE1-GL cells. The adhesive ability of CNE1-GL cells with matrix was significantly higher than those of CNE1 and AntiEzrin-CNE1-GL cells [adhesive rate: (89.38+/-6.12)% vs. (49.42+/-5.37)% and (56.94+/-4.08)%, P<0.001, P<0.05]. The invasion and migration of CNE1-GL cells were significantly higher than those of CNE1 and AntiEzrin-CNE1-GL cells (invasive cells: 107+/-11 vs. 27+/-3 and 38+/-4, P<0.001; migratory cells: 179+/-25 vs. 46+/-6 and 51+/-5, P<0.001).

CONCLUSION

EBV-LMP1 contributes to the metastasis ability of CNE1 cells by up-regulating the expression of Ezrin.

摘要

背景与目的

埃兹蛋白是一种细胞骨架相关蛋白，与肿瘤的侵袭和转移有关；我们之前的研究还表明，爱泼斯坦-巴尔病毒潜伏膜蛋白1（EBV-LMP1）可促进鼻咽癌（NPC）细胞的转移。本研究旨在探讨EBV-LMP1是否能改变埃兹蛋白的表达以及埃兹蛋白在NPC细胞转移中的作用。

方法

采用SP免疫组织化学和蛋白质印迹法检测NPC细胞系CNE1（高分化NPC细胞系）和CNE1-GL（稳定转染EBV-LMP1的CNE1细胞）中EBV-LMP1和埃兹蛋白的表达。通过细胞-基质黏附试验评估CNE1、CNE1-GL和抗埃兹蛋白-CNE1-GL（用埃兹蛋白抗体预处理的CNE1-GL细胞）细胞的黏附能力。采用Transwell法研究这些细胞系的侵袭和迁移能力。

结果

埃兹蛋白在CNE1细胞中未表达，而在CNE1-GL细胞中高表达。CNE1-GL细胞与基质的黏附能力显著高于CNE1和抗埃兹蛋白-CNE1-GL细胞[黏附率：(89.38±6.12)%对(49.42±5.37)%和(56.94±4.08)%，P<0.001，P<0.05]。CNE1-GL细胞的侵袭和迁移能力显著高于CNE1和抗埃兹蛋白-CNE1-GL细胞（侵袭细胞：107±11对27±3和38±4，P<0.001；迁移细胞：179±25对46±6和51±5，P<0.001）。