鼻咽癌中爱泼斯坦-巴尔病毒潜伏膜蛋白1（LMP1）30碱基对缺失及XhoI多态性：观察性研究的荟萃分析
The Epstein-Barr virus latent membrane protein-1 (LMP1) 30-bp deletion and XhoI-polymorphism in nasopharyngeal carcinoma: a meta-analysis of observational studies.
作者信息
da Costa Vivaldo G, Marques-Silva Ariany C, Moreli Marcos L
机构信息
Post-Graduation Program in Applied Health Sciences, Federal University of Goiás, BR 364, Km 192, Industrial Park, Jataí, Brazil.
Virology Laboratory, Federal University of Goiás, BR 364, Km 192, Industrial Park, Jataí, Brazil.
出版信息
Syst Rev. 2015 Apr 13;4:46. doi: 10.1186/s13643-015-0037-z.
BACKGROUND
Epstein-Barr virus (EBV) is considered to be closely associated with nasopharyngeal carcinoma (NPC), in which EBV-encoded latent membrane protein 1 (LMP1) was found to have an oncogenic role. However, the results published on the LMP1 polymorphism are inconsistent. In the present study, we performed a meta-analysis to determine the frequency of the associations and a more precise association between NPC and EBV LMP1 gene variants (30-bp deletion (del)/XhoI-loss).
METHODS
Eligible articles met the inclusion/exclusion criteria and were identified in the following electronic databases: PubMed, ScienceDirect, and SciELO. Consequently, the data of interest were extracted and plotted in a table to calculate the frequency and odds ratio (OR) of the outcomes of interest (30-bp del-LMP1/XhoI-loss) in patients with NPC. Study quality (Newcastle-Ottawa Scale (NOS)), publication bias, and heterogeneity were assessed.
RESULTS
Thirty-one observational studies were included with a total of 2,846 individuals (NPC, n = 1,855; control, n = 991). The risk of bias in relation to study quality evaluated by NOS was considered low. The pooled estimate of the frequency of 30-bp del-LMP1 and XhoI-loss in patients with NPC was 77% (95% confidence interval (CI): 72 to 82) and 82% (95% CI: 71 to 92), respectively. There was an association between 30-bp del-LMP1 and NPC susceptibility (OR = 2.86, 95% CI: 1.35 to 6.07, P = 0.00). Similarly, there was an association between XhoI-loss and NPC (OR = 8.5, 95% CI: 1.7 to 41, P = 0.00). However, when we analyze the co-existence of the 30-bp del-LMP1 and XhoI-loss in patients with NPC, there was no association (OR = 1.09, 95% CI: 0.06 to 18.79, P = 0.002).
CONCLUSIONS
Our results suggest an association between the 30-bp del-LMP1 and XhoI-loss with NPC susceptibility. However, our data should be interpreted with caution because the sample size was small, and there was heterogeneity between the studies. Thus, future studies are needed with adjusted estimates to simultaneously evaluate multiple factors involved in the development of NPC.
SYSTEMATIC REVIEW REGISTRATION
PROSPERO CRD42014013496 .
背景
爱泼斯坦-巴尔病毒（EBV）被认为与鼻咽癌（NPC）密切相关，其中EBV编码的潜伏膜蛋白1（LMP1）被发现具有致癌作用。然而，关于LMP1多态性发表的结果并不一致。在本研究中，我们进行了一项荟萃分析，以确定NPC与EBV LMP1基因变异（30碱基对缺失（del）/XhoI缺失）之间关联的频率以及更精确的关联。
方法
符合纳入/排除标准的合格文章在以下电子数据库中被识别：PubMed、ScienceDirect和SciELO。因此，提取了感兴趣的数据并制成表格，以计算NPC患者中感兴趣结果（30碱基对del-LMP1/XhoI缺失）的频率和比值比（OR）。评估了研究质量（纽卡斯尔-渥太华量表（NOS））、发表偏倚和异质性。
结果
纳入了31项观察性研究，共2846人（NPC患者，n = 1855；对照组，n = 991）。通过NOS评估的与研究质量相关的偏倚风险被认为较低。NPC患者中30碱基对del-LMP1和XhoI缺失频率的合并估计分别为77%（95%置信区间（CI）：72至82）和82%（95%CI：71至92）。30碱基对del-LMP1与NPC易感性之间存在关联（OR = 2.86，95%CI：1.35至6.07，P = 0.00）。同样，XhoI缺失与NPC之间存在关联（OR = 8.5，95%CI：1.7至41，P = 0.00）。然而，当我们分析NPC患者中30碱基对del-LMP1和XhoI缺失的共存情况时，没有关联（OR = 1.09，95%CI：0.06至18.79，P = 0.002）。
结论
我们的结果表明30碱基对del-LMP1和XhoI缺失与NPC易感性之间存在关联。然而，我们的数据应谨慎解释，因为样本量较小，且研究之间存在异质性。因此，未来需要进行调整估计的研究，以同时评估参与NPC发生发展的多个因素。
系统评价注册
PROSPERO CRD42014013496 。
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