Ji Huiling, Dailey Tanya L, Long Vit, Chien Edward K
Section of Maternal-Fetal Medicine, Department of Obstetrics and Gynecology, Women and Infants' Hospital of Rhode Island, Brown University, Providence, RI, USA.
Am J Obstet Gynecol. 2008 May;198(5):543.e1-9. doi: 10.1016/j.ajog.2007.11.012. Epub 2008 Feb 14.
Androgens regulate biomechanical responses in load-bearing tissues. Evidence suggests that androgens may play a role in the cervix. We hypothesized that androgens directly regulate cervical remodeling by altering both collagen structure and proteoglycan composition.
Cervical resistance was evaluated using the cervical creep method after the administration of intravaginal dihydrotestosterone or oral flutamide. Microstructural changes in collagen were evaluated by transmission electron microscopy and polarized light birefringence. Proteoglycan expression was evaluated by reverse transcription-polymerase chain reaction for the core proteins (decorin, biglycan, fibromodulin, aggrecan, versican) and fluorophore-assisted carbohydrate analysis.
Dihydrotestosterone decreased cervical resistance, whereas flutamide inhibited the decline in cervical resistance, compared with vehicle controls. Flutamide was associated with higher levels of organized collagen and increased aggrecan expression with a greater proportion of chondroitin/dermatan sulfate glycosaminoglycans. Flutamide inhibited the increase in hyaluronan.
Androgens appear to play a role in regulating cervical resistance by altering proteoglycan content. Structural analysis indicates that flutamide may alter collagen fibril organization and/or structure.
雄激素调节承重组织的生物力学反应。有证据表明雄激素可能在子宫颈中发挥作用。我们假设雄激素通过改变胶原蛋白结构和蛋白聚糖组成直接调节子宫颈重塑。
在阴道内给予二氢睾酮或口服氟他胺后，使用子宫颈蠕变方法评估子宫颈阻力。通过透射电子显微镜和偏振光双折射评估胶原蛋白的微观结构变化。通过逆转录-聚合酶链反应检测核心蛋白（核心蛋白聚糖、双糖链蛋白聚糖、纤调蛋白聚糖、聚集蛋白聚糖、多功能蛋白聚糖）以及荧光辅助碳水化合物分析评估蛋白聚糖表达。
与载体对照相比，二氢睾酮降低了子宫颈阻力，而氟他胺抑制了子宫颈阻力的下降。氟他胺与更高水平的有序胶原蛋白以及聚集蛋白聚糖表达增加相关，且硫酸软骨素/硫酸皮肤素糖胺聚糖的比例更高。氟他胺抑制了透明质酸的增加。
雄激素似乎通过改变蛋白聚糖含量在调节子宫颈阻力中发挥作用。结构分析表明氟他胺可能改变胶原纤维的组织和/或结构。
