14-3-3蛋白与单链寡脱氧核苷酸适配体的结合。
Binding of 14-3-3 proteins to a single stranded oligodeoxynucleotide aptamer.
作者信息
Stevenson Ross, Baxter Helen C, Aitken Alastair, Brown Tom, Baxter Robert L
机构信息
School of Chemistry, University of Edinburgh, Joseph Black Building, West Mains Road, Edinburgh, Scotland EH9 3JJ, UK.
出版信息
Bioorg Chem. 2008 Oct;36(5):215-9. doi: 10.1016/j.bioorg.2007.12.005. Epub 2008 Feb 14.
A synthetic library of ca. 10(13) single stranded oligodeoxynucleotides, each comprising a randomized 40mer sequence and homogeneous 10mer flanking regions, was screened for binding to recombinant human 14-3-3gamma. A single aptamer, which showed similar affinities (K(D) approximately 10(-8)M) for six isoforms of the protein, has been shown to bind to undenatured 14-3-3 protein in the cerebral spinal fluid of scrapie infected sheep.
一个约有10¹³个单链寡聚脱氧核苷酸的合成文库，每个文库包含一个随机的40聚体序列和同源的10聚体侧翼区域，用于筛选与重组人14-3-3γ的结合。已证明一种对该蛋白的六种同工型表现出相似亲和力（解离常数K(D)约为10⁻⁸M）的单适体，能与瘙痒病感染绵羊脑脊液中的未变性14-3-3蛋白结合。
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