糖尿病心肌病中尾加压素II及其受体的表达升高。
Elevated expression of urotensin II and its receptor in diabetic cardiomyopathy.
作者信息
Dai Hong-Yan, Guo Xin-Gui, Ge Zhi-Ming, Li Zhen-Hua, Yu Xiao-Jing, Tang Meng-Xiong, Zhang Yun
机构信息
Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education and Chinese Ministry of Public Health, Qilu Hospital, Shandong University, Jinan, Shandong, China.
出版信息
J Diabetes Complications. 2008 Mar-Apr;22(2):137-43. doi: 10.1016/j.jdiacomp.2006.10.008.
OBJECTIVE
Urotensin II (UII) is a somatostatin-like peptide recently identified to have several cardiovascular effects, including potent vasoactive, cardiac inotropic and chronotropic properties. Our aim was to determine the degree of expression of UII and UII receptor (UT) in the myocardium of rats with streptozotocin (STZ)-induced diabetes.
METHODS
Real-time polymerase chain reaction, Western blot, and immunohistochemistry were used to determine the degree of expression and location of UII and UT in the myocardium of STZ-induced diabetic rats.
RESULTS
UII and UT expression were significantly enhanced in the myocardium of rats with diabetes compared with healthy controls on both messenger RNA and protein levels. Both UII and UT protein expression were mainly concentrated in the cardiomyocytes, endothelial cells, cardiac fibroblasts, and smooth muscle cells of diabetic cardiomyopathy (DCM).
CONCLUSIONS
Our results suggest a possible role for the UII/UT system in the pathophysiology of DCM.
目的
尾加压素II（UII）是一种类生长抑素肽，最近被发现具有多种心血管效应，包括强大的血管活性、强心和变时特性。我们的目的是确定链脲佐菌素（STZ）诱导的糖尿病大鼠心肌中UII和UII受体（UT）的表达程度。
方法
采用实时聚合酶链反应、蛋白质印迹法和免疫组织化学法来确定STZ诱导的糖尿病大鼠心肌中UII和UT的表达程度及定位。
结果
与健康对照相比，糖尿病大鼠心肌中UII和UT在信使核糖核酸和蛋白质水平上的表达均显著增强。UII和UT蛋白表达主要集中在糖尿病性心肌病（DCM）的心肌细胞、内皮细胞、心脏成纤维细胞和平滑肌细胞中。
结论
我们的结果提示UII/UT系统在DCM病理生理学中可能发挥作用。
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