Sigala S, Bodei S, Missale C, Zani D, Simeone C, Cunico S Cosciani, Spano P F
Section of Pharmacology, Department of Biomedical Sciences and Biotechnology, University of Brescia Medical School, V.le Europa 11, 25123 Brescia, Italy.
Mol Cell Endocrinol. 2008 Mar 12;284(1-2):11-20. doi: 10.1016/j.mce.2007.12.015. Epub 2008 Jan 8.
A dysregulation of the nerve growth factor (NGF)-mediated control of prostate cell growth is associated with the malignant progression of prostate epithelial cells. Exogenous NGF induced in prostate cancer (PCa) cell lines DU145 and PC3 the expression of p75(NGFR), accompanied by a reduction of the cell malignancy. The aim of this study was to analyze the profile of NGF-regulated genes the PCa cell line DU145 by using the cDNA microarray technique. NGF treatment of DU145 cells decreased the expression of 52 known genes, while the expression of 40 known genes was increased. NGF treatment of the DU145 cell line modified the expression profile of clusters of genes involved in invasion and metastasis, in cell proliferation and apoptosis, inflammation, cell metabolism and transcriptional activity. Interestingly, NGF induced the same pattern of gene modifications in both PCa cell lines. Data presented here may help to identify gene/proteins that dispose to PCa progression and to assess future markers that could allow the development of new clinic diagnostic and therapeutical approaches.
神经生长因子（NGF）介导的前列腺细胞生长调控失调与前列腺上皮细胞的恶性进展相关。外源性NGF在前列腺癌细胞系DU145和PC3中诱导p75（NGFR）的表达，同时细胞恶性程度降低。本研究的目的是利用cDNA微阵列技术分析前列腺癌细胞系DU145中NGF调控基因的概况。用NGF处理DU145细胞可使52个已知基因的表达降低，同时40个已知基因的表达增加。用NGF处理DU145细胞系改变了参与侵袭和转移、细胞增殖和凋亡、炎症、细胞代谢及转录活性的基因簇的表达谱。有趣的是，NGF在两种前列腺癌细胞系中诱导出相同的基因修饰模式。本文提供的数据可能有助于识别与前列腺癌进展相关的基因/蛋白质，并评估未来可用于开发新的临床诊断和治疗方法的标志物。
