Schusdziarra Volker, Zimmermann Jens-Peter, Erdmann Johannes, Bader Ulrich, Schick Rafael R
Else-Kröner-Fresenius Center of Nutritional Medicine, Technical University of Munich, Germany.
Regul Pept. 2008 Apr 10;147(1-3):29-32. doi: 10.1016/j.regpep.2007.12.004. Epub 2007 Dec 28.
Feeding regulation involves both anorectic and orexigenic neuropeptides mainly located in the hypothalamus. To gain further insight into the interaction between these two groups of regulators inhibition of feeding induced by glucagon-like peptide-1 (GLP-1) was examined during stimulation of food intake by equimolar doses of ghrelin and galanin. The experiments were carried out in freely feeding rats. Intracerebroventricular (i.c.v.) injections were accomplished through stereotaxically implanted cannulae aimed at the lateral cerebral ventricle. Food intake of standard rat chow pellets was subsequently recorded for 2 h. Ghrelin and galanin stimulated food intake significantly with no difference between these two peptides. During ghrelin stimulation GLP-1 inhibited feeding in doses between 0.015 and 1.5 nmol. During galanin stimulation of food intake a ten fold higher dose (0.15 nmol) was required to inhibit food intake. In conclusion equimolar doses of i.c.v. ghrelin and galanin are similarly effective stimuli of food intake when given alone. However in combination with an anorectic neuropeptide such as GLP-1 they have substantially different potencies of feeding stimulation. Such interaction could also be of interest for therapeutic strategies involving both regulating groups of neuropeptides.
进食调节涉及主要位于下丘脑的厌食性和促食欲性神经肽。为了进一步深入了解这两组调节因子之间的相互作用，在等摩尔剂量的胃饥饿素和甘丙肽刺激食物摄入期间，研究了胰高血糖素样肽-1（GLP-1）诱导的进食抑制作用。实验在自由进食的大鼠中进行。通过立体定位植入的套管向侧脑室进行脑室内（i.c.v.）注射。随后记录2小时内标准大鼠饲料颗粒的食物摄入量。胃饥饿素和甘丙肽均显著刺激食物摄入，这两种肽之间无差异。在胃饥饿素刺激期间，GLP-1以0.015至1.5 nmol的剂量抑制进食。在甘丙肽刺激食物摄入期间，需要高十倍的剂量（0.15 nmol）来抑制食物摄入。总之，单独给予等摩尔剂量的脑室内胃饥饿素和甘丙肽时，它们作为食物摄入的刺激物效果相似。然而，与厌食性神经肽如GLP-1联合使用时，它们对进食刺激的效力有很大差异。这种相互作用对于涉及这两组神经肽调节的治疗策略也可能具有重要意义。
