弗氏链霉菌木聚糖酶基因在大肠杆菌中的克隆以及利用噬菌体λ诱导裂解来释放克隆酶。

Cloning of xylanase gene of Streptomyces flavogriseus in Escherichia coli and bacteriophage lambda-induced lysis for the release of cloned enzyme.

作者信息

Srivastava R, Ali S S, Srivastava B S

机构信息

Division of Microbial Genetics, Central Drug Research Institute, Lucknow, India.

出版信息

FEMS Microbiol Lett. 1991 Mar 1;62(2-3):201-5. doi: 10.1016/0378-1097(91)90158-7.

DOI:10.1016/0378-1097(91)90158-7

PMID:1828240

Abstract

The xylanase gene of Streptomyces flavogriseus was cloned in pUC8 plasmid and expressed in Escherichia coli lysogenic for lambda cI857. lambda-Induced lysis of E. coli at 42 degrees C allowed efficient release of cloned enzyme activity in extracellular environment. The xylanase gene was located in the 0.8-kb HindIII fragment and coded for 18,000 Mr xylanase.

摘要

黄色链霉菌的木聚糖酶基因被克隆到pUC8质粒中，并在携带λcI857的溶原性大肠杆菌中表达。42℃时λ诱导的大肠杆菌裂解使得克隆的酶活性能够在细胞外环境中有效释放。木聚糖酶基因位于0.8kb的HindIII片段中，编码18,000分子量的木聚糖酶。