西格玛蛋白的印记：重新审视
Footprint of the sigma protein: a re-examination.
作者信息
Wellman A, Meares C F
机构信息
Chemistry Department, University of California, Davis 95616.
出版信息
Biochem Biophys Res Commun. 1991 May 31;177(1):140-4. doi: 10.1016/0006-291x(91)91959-g.
Escherichia coli RNA Polymerase is a multi-subunit enzyme that catalyzes RNA synthesis, using DNA as a template. The sigma subunit of this enzyme plays an important role in the recognition of promoter sites on DNA. Using DNase I footprinting, Utpala Ramesh and Claude F. Meares [(1989) Biochem. Biophys. Res. Comm. 160, 121-125] reported that in the absence of the other subunits, sigma binds specifically to the bacteriophage lambda PR promoter DNA sequence. We are unable to reproduce that result.
大肠杆菌RNA聚合酶是一种多亚基酶，它以DNA为模板催化RNA合成。该酶的σ亚基在识别DNA上的启动子位点中起重要作用。Utpala Ramesh和Claude F. Meares [（1989年）《生物化学与生物物理学研究通讯》160, 121 - 125] 使用DNase I足迹法报道，在没有其他亚基的情况下，σ特异性结合噬菌体λ PR启动子DNA序列。我们无法重现该结果。
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