Fadel Sanaa, Eley Adrian
Henry Wellcome Laboratories for Medical Research, Unit of Infection and Immunity, School of Medicine and Biomedical Sciences, University of Sheffield Medical School, Beech Hill Road, Sheffield S10 2RX, UK.
J Med Microbiol. 2008 Mar;57(Pt 3):261-266. doi: 10.1099/jmm.0.47237-0.
Lipopolysaccharide (LPS) is a major surface component of Chlamydia trachomatis, as with all Gram-negative bacteria. The effect of C. trachomatis LPS on C. trachomatis infectivity of human epithelial cells was investigated. C. trachomatis LPS and C. trachomatis LPS antibody significantly reduced infectivity, mostly in a dose-dependent manner. As the structure of LPS in C. trachomatis is simple and consists only of lipid A and 3-deoxy-D-manno-octulosonic acid (Kdo), we investigated whether lipid A or Kdo was inhibitory to chlamydial infectivity. Polymyxin B, as a lipid A inhibitor, and Kdo considerably reduced C. trachomatis infectivity. With all the LPS inhibitors used, there was greater inhibition against serovar E than serovar LGV. These results suggest a role for LPS in chlamydial infectivity. Elucidation of how LPS acts in infectivity and identification of host-cell receptors would help in understanding pathogenicity.
与所有革兰氏阴性菌一样，脂多糖（LPS）是沙眼衣原体的主要表面成分。研究了沙眼衣原体LPS对人上皮细胞沙眼衣原体感染性的影响。沙眼衣原体LPS和沙眼衣原体LPS抗体显著降低感染性，大多呈剂量依赖性。由于沙眼衣原体中LPS的结构简单，仅由脂质A和3-脱氧-D-甘露糖辛酸（Kdo）组成，我们研究了脂质A或Kdo是否对衣原体感染性有抑制作用。作为脂质A抑制剂的多粘菌素B和Kdo显著降低了沙眼衣原体的感染性。使用的所有LPS抑制剂对血清型E的抑制作用比对血清型LGV的抑制作用更强。这些结果表明LPS在衣原体感染性中起作用。阐明LPS如何在感染性中发挥作用以及鉴定宿主细胞受体将有助于理解致病性。
