锤头结构再探讨：新结构是否符合旧的生化数据？
Hammerhead redux: does the new structure fit the old biochemical data?
作者信息
Nelson Jennifer A, Uhlenbeck Olke C
机构信息
Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, Illinois 60208, USA.
出版信息
RNA. 2008 Apr;14(4):605-15. doi: 10.1261/rna.912608. Epub 2008 Feb 20.
Abstract
The cleavage rates of 78 hammerhead ribozymes containing structurally conservative chemical modifications were collected from the literature and compared to the recently determined crystal structure of the Schistosoma mansoni hammerhead. With only a few exceptions, the biochemical data were consistent with the structure, indicating that the new structure closely resembles the transition state of the reaction. Since all the biochemical data were collected on minimal hammerheads that have a very different structure, the minimal hammerhead must be dynamic and occasionally adopt the quite different extended structure in order to cleave.
摘要
从文献中收集了78个含有结构保守化学修饰的锤头状核酶的切割率，并与最近测定的曼氏血吸虫锤头状核酶的晶体结构进行了比较。除了少数例外，生化数据与该结构一致，这表明新结构与反应的过渡态非常相似。由于所有生化数据都是在结构差异很大的最小锤头状核酶上收集的，因此最小锤头状核酶一定是动态的，并且偶尔会采用截然不同的延伸结构以便进行切割。
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本文引用的文献
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