嗜中性粒细胞吞噬过程中金黄色葡萄球菌基因表达的分析

Analysis of Staphylococcus aureus gene expression during PMN phagocytosis.

作者信息

Voyich Jovanka M, Sturdevant Dan E, DeLeo Frank R

机构信息

Department of Veterinary Molecular Biology, Montana State University, Bozeman, MT, USA.

出版信息

Methods Mol Biol. 2008;431:109-22. doi: 10.1007/978-1-60327-032-8_9.

DOI:10.1007/978-1-60327-032-8_9

PMID:18287751

Abstract

Staphylococcus aureus is a leading cause of human infections worldwide and causes a variety of diseases ranging in severity from mild to life-threatening. The ability of S. aureus to cause disease is based in part on its ability to subvert the innate immune system. Advances in genome-wide analysis of host-pathogen interactions have provided the necessary tools to investigate molecular factors that directly contribute to S. aureus pathogenesis. This chapter describes methods to analyze gene expression in S. aureus during interaction with human neutrophils.

摘要

金黄色葡萄球菌是全球人类感染的主要病因，可引发从轻度到危及生命的各种疾病。金黄色葡萄球菌致病的能力部分基于其破坏固有免疫系统的能力。宿主-病原体相互作用的全基因组分析进展为研究直接导致金黄色葡萄球菌发病机制的分子因素提供了必要工具。本章介绍了在金黄色葡萄球菌与人类中性粒细胞相互作用过程中分析其基因表达的方法。