Takahashi Kayo, Tamura Yasuhisa, Watanabe Yasuyoshi, Långström Bengt, Bergström Mats
Molecular Probe Dynamics Laboratory, Molecular Imaging Research Program, RIKEN, Kobe, Japan.
Neuroreport. 2008 Mar 5;19(4):431-5. doi: 10.1097/WNR.0b013e3282f7cdb7.
In a previous study, we demonstrated that androgenic-anabolic steroids increased aromatase expression in the bed nucleus of stria terminalis and preoptic area in rat brain, as evaluated using autoradiography with [11C]vorozole, a potential positron emission tomography tracer for aromatase. In this study, we explored whether the increase in aromatase binding is mediated via androgen receptors and whether this increase occurs in neurons or glial cells. Rats were given nandrolone decanoate (15 mg/kg body weight once every 3 days) and flutamide (20 mg/kg/day) alone or in combination for 20 days. Results indicated a significant increase of [11C]vorozole binding by nandrolone decanoate in the bed nucleus of the stria terminalis and preoptic area, as in our previous study. Flutamide treatment, on the other hand, decreased [11C]vorozole binding in the bed nucleus of the stria terminalis, preoptic area, and medial amygdala. Immunohistochemical examination demonstrated that upregulation of aromatase expression occurred in neurons. Our findings suggest that aromatase is regulated through an androgen receptor-mediated system. This aromatase-specific tracer and the positron emission tomography technique could be useful for exploring the role of aromatase in anabolic androgenic steroids abusers.
在之前的一项研究中，我们证明，使用[11C]伏洛唑（一种潜在的芳香化酶正电子发射断层扫描示踪剂）进行放射自显影评估时，雄激素同化类固醇会增加大鼠脑内终纹床核和视前区的芳香化酶表达。在本研究中，我们探究了芳香化酶结合的增加是否通过雄激素受体介导，以及这种增加是否发生在神经元或神经胶质细胞中。给大鼠单独或联合给予癸酸诺龙（15 mg/kg体重，每3天一次）和氟他胺（20 mg/kg/天），持续20天。结果表明，与我们之前的研究一样，癸酸诺龙使终纹床核和视前区的[11C]伏洛唑结合显著增加。另一方面，氟他胺治疗降低了终纹床核、视前区和杏仁核内侧的[11C]伏洛唑结合。免疫组织化学检查表明，芳香化酶表达上调发生在神经元中。我们的研究结果表明，芳香化酶是通过雄激素受体介导的系统调节的。这种芳香化酶特异性示踪剂和正电子发射断层扫描技术可能有助于探究芳香化酶在合成代谢雄激素类固醇滥用者中的作用。
