The time-space correlation method for measurement of erythrocyte velocity in microvessels using a CCD linear image sensor.

A new method for measuring erythrocyte velocity in the microvascular system was developed. A correlation function varying both time and distance, "time-space correlation method," was used in order to improve the time correlation and the space correlation methods. A CCD linear image sensor installed at the center of the focal plane of a camera was used to convert the passage of erythrocytes through the microvascular system to electrical signals via intravital microscopy. The method was applied to small vessels in the mesentery of rats. Using the present method, the time required for the measurement and the length of the vessel necessary for the measurement have been reduced, as compared with other methods.