Nouri A M, Bergbaum A, Lederer E, Crosby D, Shamsa A, Oliver R T
Department of Medical Oncology, Royal London Hospital, Whitechapel, U.K.
Eur J Cancer. 1991;27(5):608-12. doi: 10.1016/0277-5379(91)90241-5.
This paper reports the first example of tumour infiltrating lymphocytes (TILs) and a tumour cell line from the same individual and analyses their characteristics. The tumour cell line (CAT), derived from a patient with well-differentiated (G3pTa) TCC, has been in culture for 24 months and subcultured more than 100 times. Epithelial origin was established by electronmicroscopy and use of a range of monoclonal antibodies (Mabs) against cytokeratins. The TILs isolated from the same tumour expressed all the phenotypic characteristics of normal activated T cells and demonstrated low levels of cytotoxicity against the autologous tumour line (CAT). Comparison of cell surface molecules of these cells revealed the loss of HLA-B7, B44 and Bw6 from the CAT cells whilst maintaining HLA-A2, A3 and Bw4. Karyotypic analysis demonstrated three rearranged chromosomes (between chromosomes 4 and 11, 10 and 13, 11 and 17) on CAT cells. The potential that study of paired autologous tumour cells and TILs in culture offers for studying the role of MHC antigens in tumour rejection and the impact of different approaches to correcting the defect are reviewed.
本文报道了首例来自同一个体的肿瘤浸润淋巴细胞（TILs）和肿瘤细胞系，并分析了它们的特征。该肿瘤细胞系（CAT）源自一名高分化（G3pTa）移行细胞癌患者，已培养24个月，传代100多次。通过电子显微镜和一系列针对细胞角蛋白的单克隆抗体（Mabs）确定其上皮来源。从同一肿瘤中分离出的TILs表现出正常活化T细胞的所有表型特征，并对自体肿瘤细胞系（CAT）表现出低水平的细胞毒性。对这些细胞的细胞表面分子进行比较发现，CAT细胞失去了HLA - B7、B44和Bw6，同时保留了HLA - A2、A3和Bw4。核型分析显示CAT细胞有三条重排染色体（位于4号和11号、10号和13号、11号和17号染色体之间）。本文还综述了在培养中研究配对自体肿瘤细胞和TILs对于研究MHC抗原在肿瘤排斥中的作用以及不同纠正缺陷方法的影响的潜力。
