Shen Yu-jia, Hong Yuan-fan, Hong Qing, Jiang Xin, Li Shun-peng
Key Laboratory of Microbiological Engineering Agricultural Environment, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing 210095, China.
Huan Jing Ke Xue. 2007 Dec;28(12):2833-7.
A bacteria strain XSP-1 capable of utilizing phoxim as sole carbon source was isolated from sludge collected from a pesticide manufacturer. It was preliminarily identified as Delftia sp. according to its physiological-biochemical analysis and the similarity analysis of its 16S rRNA gene sequence (GenBank Accession No. EF061135). Strain XSP-1 could degrade 100 mg/L phoxim within 7 h completely. The optimal pH and temperature for degradation were 7.0 and 35 degrees C respectively. The degrading rate showed a positive correlation to the initial inoculum size. Strain XSP-1 also showed good degrading performance against methyl parathion, chlorpyrifos and fenitrothion. PCR detection with degenerated primers designed according to the conserved sequences of reported mpd gene did not find target band, but it needs further study to verify whether strain XSP-1 harbors a new mpd gene.
从一家农药生产厂收集的污泥中分离出一株能够以辛硫磷作为唯一碳源的细菌菌株XSP-1。根据其生理生化分析以及16S rRNA基因序列（GenBank登录号：EF061135）的相似性分析，初步鉴定为代尔夫特菌属。菌株XSP-1能够在7小时内完全降解100 mg/L的辛硫磷。降解的最适pH值和温度分别为7.0和35℃。降解速率与初始接种量呈正相关。菌株XSP-1对甲基对硫磷、毒死蜱和杀螟硫磷也表现出良好的降解性能。根据已报道的mpd基因保守序列设计的简并引物进行PCR检测未发现目标条带，但菌株XSP-1是否含有新的mpd基因还需进一步研究验证。
