Qin Zhi, Ren Nan-qi, Li Jian-zheng
School of Chemical and Environmental Engineering, Harbin University of Science and Technology, Harbin 150040, China.
Huan Jing Ke Xue. 2007 Dec;28(12):2843-8.
Hydrogen producing strain Ethanoligenens sp. B49 was inoculated into activated sludge of continuous stirred tank reactor (CSTR)to bioaugment hydrogen production. Hydrogen production capacities, compositions of fermentation products and pH value before and after bioaugmentation were investigated. When organic loading rate was 12 kg/(m3 x d), bioaugmentation of hydrogen producing strain enhanced hydrogen production rate and improved the composition of fermentation products significantly. After bioaugmentation, hydrogen production rate increased from 3.6 mmol/(kg x d) to 5.7 mmol/(kg x d), which was 1.5 times as that before bioaugmentation. Before bioaugmentation, average concentration of ethanol, acetic acid and propionic acid were 6.8 mmol/L, 5.3 mmol/L, 4.8 mmol/L respectively, while after bioaugmentation, those were 10.5 mmol/L, 7.5 mmol/L and 1.7 mmol/L respectively. Ethanol and acetic acid accounted for 86.8% in total fermentative products after bioaugmentation, while only 72% before bioaugmentation. pH value of effluent dropped from 4.5-4.7 to 4.3. Bioaugmentation of hydrogen producing strain is helpful to promote the formation of ethanol-type fermentation in low organic loading rate.
产氢菌株嗜乙醇杆菌属B49接种到连续搅拌槽式反应器（CSTR）的活性污泥中以进行生物强化产氢。研究了生物强化前后的产氢能力、发酵产物组成和pH值。当有机负荷率为12 kg/(m³·d)时，产氢菌株的生物强化提高了产氢速率，并显著改善了发酵产物的组成。生物强化后，产氢速率从3.6 mmol/(kg·d)提高到5.7 mmol/(kg·d)，是生物强化前的1.5倍。生物强化前，乙醇、乙酸和丙酸的平均浓度分别为6.8 mmol/L、5.3 mmol/L、4.8 mmol/L，而生物强化后分别为10.5 mmol/L、7.5 mmol/L和1.7 mmol/L。生物强化后，乙醇和乙酸在总发酵产物中占86.8%，而生物强化前仅占72%。出水pH值从4.5 - 4.7降至4.3。产氢菌株的生物强化有助于在低有机负荷率下促进乙醇型发酵的形成。
