Samuelsson Jörgen, Arnell Robert, Diesen Jarle S, Tibbelin Julius, Paptchikhine Alexander, Fornstedt Torgny, Sjöberg Per J R
Department of Physical and Analytical Chemistry, Uppsala University, BMC, Box 577, SE-751 23 Uppsala, Sweden.
Anal Chem. 2008 Mar 15;80(6):2105-12. doi: 10.1021/ac702399a. Epub 2008 Feb 22.
The tracer-pulse method provides the real adsorption data points directly from simple, straightforward calculations and is therefore a superior method for multicomponent adsorption isotherm determination in HPLC. Only one important problem has restricted its use so far: the tracer peaks are invisible using any conventional detection principle. We present a solution to this problem with an approach with a firm base in analytical chemistry, utilizing stable isotopes and mass spectrometric detection. The new approach was used for the determination of binary adsorption isotherms, and a systematic investigation was made of its main sources of error. With this modification, the tracer method can be a prime choice for future characterizations of multicomponent separation systems and of competitive drug binding studies.
示踪脉冲法可直接通过简单、直接的计算得出真实的吸附数据点，因此是测定高效液相色谱中多组分吸附等温线的一种优越方法。到目前为止，只有一个重要问题限制了它的应用：使用任何传统检测原理都无法看到示踪峰。我们基于分析化学，利用稳定同位素和质谱检测，提出了一个解决该问题的方案。这种新方法用于二元吸附等温线的测定，并对其主要误差来源进行了系统研究。通过这种改进，示踪法可能成为未来多组分分离系统表征和竞争性药物结合研究的首选方法。
