采用微径高效液相色谱法和进样生成梯度法测定血浆中的替考拉宁。

Determination of teicoplanin in plasma using microbore high-performance liquid chromatography and injection-generated gradients.

作者信息

Taylor R B, Reid R G, Gould I M

机构信息

Robert Gordon's Institute of Technology, Schoolhill, Aberdeen, U.K.

出版信息

J Chromatogr. 1991 Feb 15;563(2):451-7. doi: 10.1016/0378-4347(91)80055-h.

DOI:10.1016/0378-4347(91)80055-h

PMID:1829090

Abstract

A reversed-phase isocratic high-performance liquid chromatographic method for the determination of total teicoplanin in plasma is reported. The method developed uses a bracketing injection technique in conjunction with large injection volumes on a 1 mm diameter column to form a limited injection-generated gradient. The chromatography yields adequate resolution among all the major components for individual quantitation and also allows quantitation of total teicoplanin in plasma using ultraviolet detection. Pretreatment is by solid-phase extraction which uses C8 Bond Elut cartridges and gives effective clean up from endogenous materials. The method offers a faster and simplified means to determine total teicoplanin in plasma than those previously reported, and has a detection limit of 50 ng/ml.

摘要

报道了一种反相等度高效液相色谱法测定血浆中替考拉宁总量。所开发的方法采用括弧进样技术，并结合在1毫米直径的色谱柱上进样大体积样品，以形成有限的进样产生的梯度。该色谱法在所有主要成分之间产生了足够的分离度，可进行单独定量，并且还允许使用紫外检测对血浆中的替考拉宁总量进行定量。预处理采用固相萃取，使用C8 Bond Elut柱，能有效去除内源性物质。该方法提供了一种比先前报道的方法更快、更简便的测定血浆中替考拉宁总量的手段，检测限为50纳克/毫升。