Revazishvili T, Rajanna C, Bakanidze L, Tsertsvadze N, Imnadze P, O'Connell K, Kreger A, Stine O C, Morris J G, Sulakvelidze A
Emerging Pathogens Institute and Department of Molecular Genetics and Microbiology, University of Florida College of Medicine, Gainesville, FL 32610, USA.
Clin Microbiol Infect. 2008 May;14(5):429-36. doi: 10.1111/j.1469-0691.2008.01953.x. Epub 2008 Feb 21.
Forty Yersinia pestis isolates from endemic foci of plague in the Republic of Georgia, and six Y. pestis isolates from neighbouring former Soviet Union countries, were analysed for their biochemical and phenotypic properties, and their genetic relatedness was compared with Y. pestis strains KIM and CO92 by pulsed-field gel electrophoresis (PFGE). In addition, 11 Y. pestis isolates from the USA, together with published nucleotide sequences from Y. pestis strains KIM, CO92 and 91001, were compared with the 46 isolates in the present collection using multilocus sequence typing (MLST), based on sequence data for the 16S rRNA, hsp60, glnA, gyrB, recA, manB, thrA and tmk loci. Four virulence gene loci (caf1, lcrV, psaA and pla) were also sequenced and analysed. Two sequence types (ST1 and ST2), which differed by a single nucleotide, were identified by MLST. With the exception of a single isolate (771G), all of the Georgian Y. pestis isolates belonged to ST2. PFGE also grouped the Georgian Y. pestis isolates separately from the non-Georgian isolates. Overall, PFGE discriminated the Y. pestis isolates more effectively than MLST. The sequences of three of the four virulence genes (lcrV, psaA and pla) were identical in all Georgian and non-Georgian isolates, but the caf1 locus was represented by two allele types, with caf1 NT1 being associated with the non-Georgian isolates and caf1 NT2 being associated with the Georgian isolates. These results suggest that Georgian Y. pestis isolates are of clonal origin.
对从格鲁吉亚共和国鼠疫疫区分离出的40株鼠疫耶尔森菌以及从邻近的前苏联国家分离出的6株鼠疫耶尔森菌进行了生化和表型特性分析，并通过脉冲场凝胶电泳（PFGE）将它们的遗传相关性与鼠疫耶尔森菌菌株KIM和CO92进行了比较。此外，利用多位点序列分型（MLST），基于16S rRNA、hsp60、glnA、gyrB、recA、manB、thrA和tmk基因座的序列数据，将从美国分离出的11株鼠疫耶尔森菌与已发表的鼠疫耶尔森菌菌株KIM、CO92和91001的核苷酸序列一起与本研究收集的46株分离株进行了比较。还对四个毒力基因座（caf1、lcrV、psaA和pla）进行了测序和分析。通过MLST鉴定出两种仅相差一个核苷酸的序列类型（ST1和ST2）。除了一个分离株（771G）外，所有格鲁吉亚鼠疫耶尔森菌分离株都属于ST2。PFGE也将格鲁吉亚鼠疫耶尔森菌分离株与非格鲁吉亚分离株分开聚类。总体而言，PFGE比MLST能更有效地鉴别鼠疫耶尔森菌分离株。在所有格鲁吉亚和非格鲁吉亚分离株中，四个毒力基因中的三个（lcrV、psaA和pla）的序列是相同的，但caf1基因座由两种等位基因类型代表，caf1 NT1与非格鲁吉亚分离株相关，caf1 NT2与格鲁吉亚分离株相关。这些结果表明格鲁吉亚鼠疫耶尔森菌分离株起源于克隆。
