Ikegami Tohru, Tomomatsu Kouki, Takubo Hirotaka, Horie Kanta, Tanaka Nobuo
Department of Biomolecular Engineering, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan.
J Chromatogr A. 2008 Mar 14;1184(1-2):474-503. doi: 10.1016/j.chroma.2008.01.075. Epub 2008 Feb 3.
Hydrophilic interaction chromatography (HILIC) is important for the separation of highly polar substances including biologically active compounds, such as pharmaceutical drugs, neurotransmitters, nucleosides, nucleotides, amino acids, peptides, proteins, oligosaccharides, carbohydrates, etc. In the HILIC mode separation, aqueous organic solvents are used as mobile phases on more polar stationary phases that consist of bare silica, and silica phases modified with amino, amide, zwitterionic functional group, polyols including saccharides and other polar groups. This review discusses the column efficiency of HILIC materials in relation to solute and stationary phase structures, as well as comparisons between particle-packed and monolithic columns. In addition, a literature review consisting of 2006-2007 data is included, as a follow up to the excellent review by Hemström and Irgum.
亲水作用色谱法（HILIC）对于分离包括生物活性化合物在内的高极性物质非常重要，这些生物活性化合物如药物、神经递质、核苷、核苷酸、氨基酸、肽、蛋白质、寡糖、碳水化合物等。在HILIC模式分离中，水相有机溶剂被用作流动相，固定相则采用更具极性的材料，包括裸硅胶以及用氨基、酰胺、两性离子官能团、多元醇（包括糖类）和其他极性基团修饰的硅胶相。本综述讨论了HILIC材料的柱效与溶质和固定相结构的关系，以及颗粒填充柱和整体柱之间的比较。此外，还纳入了一篇涵盖2006 - 2007年数据的文献综述，作为对Hemström和Irgum出色综述的跟进。
