Sendag Sait, Cetin Yunus, Alan Muhammet, Hadeler Klaus-Gerd, Niemann Heiner
Department of Obstetrics and Gynaecology, Faculty of Veterinary Medicine, University of Yuzuncu Yil, 65080 Kampus Van, Turkey.
Anim Reprod Sci. 2008 Jun;106(1-2):208-14. doi: 10.1016/j.anireprosci.2008.01.007. Epub 2008 Jan 18.
The goal of the present study was to compare the ovarian response, oocyte yields per animal, and the morphological quality of oocytes collected by ultrasound guided follicular aspiration from Holstein cows treated either with FSH or eCG. Twenty four normal cyclic, German Holstein cows were randomly divided into two groups. Fourteen cows received 3000 IU eCG on day-4 prior to ovum pick-up (OPU) (day 0), 2 days later (day-2), 625 microg cloprostenol was administered. On day-1 GnRH was administered i.m. and 24h later OPU (day 0) was performed. In ten cows a total dose of 500 IU follicle stimulating hormone (Pluset) was administered intramuscularly in a constant dosage for 4 days with intervals of 12h, starting on day-5. Luteolysis was induced by application of 625 microg cloprostenol on day-2. On day-1 (24h after the last FSH treatment) GnRH was administered i.m. and 24h later OPU (day 0) was performed. Ovarian follicles were visualized on the ultrasound monitor, counted and recorded. All visible antral follicles were punctured. Recovered oocytes were graded morphologically based on the cumulus investment. Average follicle number in ovaries was higher in FSH group than eCG group (p<0.05). Oocyte yields per animal did not differ between FSH and eCG groups. The proportion of grade A oocytes was higher in the FSH group in the than eCG group (p<0.05). Likewise, rate of grade C oocytes in FSH group were lower than eCG group (p<0.05). In conclusion, these results suggest that ovarian response, follicle number in ovaries and oocyte quality are affected by the type of gonadotropin and FSH is better alternative than eCG for OPU treatment.
本研究的目的是比较用促卵泡素（FSH）或孕马血清促性腺激素（eCG）处理的荷斯坦奶牛，经超声引导卵泡抽吸术采集的卵巢反应、每头动物的卵母细胞产量以及卵母细胞的形态质量。24头正常发情周期的德国荷斯坦奶牛被随机分为两组。14头奶牛在采卵（OPU，第0天）前4天（第 - 4天）接受3000 IU eCG，2天后（第 - 2天），给予625微克氯前列醇。在第 - 1天肌肉注射促性腺激素释放激素（GnRH），24小时后进行OPU（第0天）。10头奶牛从第 - 5天开始，以恒定剂量肌肉注射总剂量为500 IU的促卵泡激素（Pluset），持续4天，间隔12小时。在第 - 2天应用625微克氯前列醇诱导黄体溶解。在第 - 1天（最后一次FSH治疗后24小时）肌肉注射GnRH，24小时后进行OPU（第0天）。在超声监测仪上观察、计数并记录卵巢卵泡。穿刺所有可见的窦状卵泡。根据卵丘包裹情况对回收的卵母细胞进行形态学分级。FSH组卵巢中的平均卵泡数高于eCG组（p<0.05）。FSH组和eCG组每头动物的卵母细胞产量没有差异。FSH组A级卵母细胞的比例高于eCG组（p<0.05）。同样，FSH组C级卵母细胞的比例低于eCG组（p<0.05）。总之，这些结果表明，卵巢反应、卵巢中的卵泡数量和卵母细胞质量受促性腺激素类型的影响，对于OPU治疗，FSH是比eCG更好的选择。
