Greisler H P, Ellinger J, Henderson S C, Shaheen A M, Burgess W H, Kim D U, Lam T M
Loyola University Medical Center, Maywood, IL 60153.
J Vasc Surg. 1991 Jul;14(1):10-23. doi: 10.1067/mva.1991.27418.
We previously reported that biomaterials differentially induced macrophages to secrete growth factors that mediate reendothelialization. The present study evaluates the effect of an atherogenic diet on macrophage/biomaterial interactions. Female New Zealand white rabbits were fed an atherogenic diet. Peritoneal macrophages were harvested from these as well as rabbits fed a normal diet and cultured in Minimum Essential Medium with platelet-poor serum. Dacron or polyglactin 910 were added to two of three conditions of both cell groups in passage 2. Conditioned media were collected weekly through week 15. Mitogenicity assays were performed with quiescent mouse embryonal (BALB/c3T3) fibroblasts, atherosclerotic rabbit aortic smooth muscle cells, and murine capillary lung (LE-II) endothelial cells. Mitogenic activity was assayed by scintillation counting of tritiated thymidine incorporation into deoxyribonucleic acid (DNA). Results showed increased mitogenic activity released by macrophages from atherosclerotic rabbits, in the absence of prosthetic material, when assayed against every cell line. In normal diet macrophages, polyglactin 910 stimulated mitogen release for every cell line, and Dacron yielded minimal mitogen release. In lipid diet macrophages polyglactin 910 slightly increased mitogen release for all three cell lines, whereas Dacron resulted in stimulation of DNA synthesis in smooth muscle cells and BALB/c3T3 cells but less DNA synthesis in LE-II cells than in control, no graft material, media. Western blotting demonstrated immunoreactivity to basic fibroblast growth factor in media from normal diet macrophages but only in the presence of polyglactin 910 or Dacron. Radioimmunoassay for platelet-derived growth factor B chain was negative in all groups, and polymerase chain reaction techniques to amplify transforming growth factor-beta messenger ribonucleic was negative. These data demonstrate the effect of in vivo dietary manipulation on macrophage activation as well as the effect of an atherogenic diet in modulating macrophage/biomaterial interactions. Additionally, different biomaterials differentially induce macrophages to release factors that stimulate and inhibit growth.
我们之前报道过，生物材料能以不同方式诱导巨噬细胞分泌介导再内皮化的生长因子。本研究评估致动脉粥样化饮食对巨噬细胞/生物材料相互作用的影响。给雌性新西兰白兔喂食致动脉粥样化饮食。从这些兔子以及喂食正常饮食的兔子中采集腹膜巨噬细胞，并在含贫血小板血清的最低必需培养基中培养。在第2代时，将涤纶或聚乙交酯丙交酯910添加到两个细胞组的三种条件中的两种。每周收集条件培养基，直至第15周。用静止的小鼠胚胎（BALB/c3T3）成纤维细胞、动脉粥样硬化兔主动脉平滑肌细胞和鼠肺毛细血管（LE-II）内皮细胞进行促有丝分裂活性测定。通过闪烁计数法测定氚标记胸腺嘧啶掺入脱氧核糖核酸（DNA）来检测促有丝分裂活性。结果显示，在无假体材料的情况下，针对每种细胞系检测时，来自动脉粥样硬化兔子的巨噬细胞释放的促有丝分裂活性增加。在正常饮食的巨噬细胞中，聚乙交酯丙交酯910刺激每种细胞系释放促有丝分裂原，而涤纶产生的促有丝分裂原释放极少。在高脂饮食的巨噬细胞中，聚乙交酯丙交酯910使所有三种细胞系的促有丝分裂原释放略有增加，而涤纶导致平滑肌细胞和BALB/c3T3细胞中的DNA合成受到刺激，但LE-II细胞中的DNA合成比对照（无移植材料的培养基）少。蛋白质印迹法显示，正常饮食巨噬细胞的培养基中对碱性成纤维细胞生长因子有免疫反应性，但仅在存在聚乙交酯丙交酯910或涤纶的情况下。血小板衍生生长因子B链的放射免疫测定在所有组中均为阴性，用于扩增转化生长因子-β信使核糖核酸的聚合酶链反应技术也为阴性。这些数据证明了体内饮食调控对巨噬细胞活化的影响，以及致动脉粥样化饮食在调节巨噬细胞/生物材料相互作用方面的影响。此外，不同的生物材料以不同方式诱导巨噬细胞释放刺激和抑制生长的因子。
