Ericson Karen L, Maloney Vincent M, Mahuren J Dennis, Coburn Stephen P, Degenhardt Thorsten P
Department of Chemistry, Indiana University-Purdue University Fort Wayne, 2101 E. Coliseum Boulevard, Fort Wayne, IN 46805-1499, USA.
Bioorg Med Chem Lett. 2008 Mar 15;18(6):1845-8. doi: 10.1016/j.bmcl.2008.02.013. Epub 2008 Feb 10.
Cation-exchange HPLC analysis of urine from dogs given large daily doses of pyridoxamine revealed an unidentified metabolite hypothesized to be N-methylpyridoxamine. Identity was established by N-methylpyridoxamine synthesis and HPLC comparison to the canine metabolite. Compound synthesis was confirmed by IR, NMR, UV-vis and emission spectroscopy. It seems to have less fluorescent character than other routinely-measured vitamin B(6) metabolites. Upon administration of substantial pyridoxamine doses, N-methylpyridoxamine appears to be a quantifiable canine urine metabolite, although, at either pharmacological or dietary pyridoxamine intakes, its relevance to vitamin B(6) metabolism in other species, including humans, is not yet determined.
