Jääskeläinen Anne J, Piiparinen Heli, Lappalainen Maija, Vaheri Antti
Department of Virology, Haartman Institute, FIN-00014 University of Helsinki, Finland.
J Clin Virol. 2008 Jun;42(2):172-5. doi: 10.1016/j.jcv.2008.01.003. Epub 2008 Mar 4.
A multiplex-PCR and microarray-based method was designed for detection of eight herpesviruses from clinical specimens.
To improve the method, especially for detection of herpes simplex (HSV) and varicella-zoster viruses (VZV), and to update and validate the method using positive cerebrospinal fluid (CSF) samples.
A new primer pair for HSV-PCR and four detection oligonucleotides for HSVs were designed. Two new detection oligonucleotides for VZV and additional oligonucleotides for CMV, EBV, HHV-6 and -7 were designed. The new and previous multiplex-PCR and microarray method were tested in parallel with dilution series of commercial herpesvirus DNAs and 20 CSF specimens positive for HSV-1, HSV-2, or VZV.
New method was more sensitive for detection of HSVs and both two new detection oligonucleotides for VZV functioned well at low levels of viral DNA.
The revised HSV-PCR and new HSV- and VZV-oligonucleotides were found to function well and be more sensitive, thereby increasing reliability of the method.
设计了一种基于多重聚合酶链反应（PCR）和微阵列的方法，用于从临床标本中检测八种疱疹病毒。
改进该方法，特别是用于检测单纯疱疹病毒（HSV）和水痘-带状疱疹病毒（VZV），并使用阳性脑脊液（CSF）样本更新和验证该方法。
设计了一对用于HSV-PCR的新引物对和四种用于HSV的检测寡核苷酸。设计了两种用于VZV的新检测寡核苷酸以及用于巨细胞病毒（CMV）、EB病毒（EBV）、人类疱疹病毒6型（HHV-6）和7型（HHV-7）的其他寡核苷酸。将新的和先前的多重PCR和微阵列方法与商业疱疹病毒DNA的稀释系列以及20份HSV-1、HSV-2或VZV阳性的脑脊液标本进行平行测试。
新方法对HSV的检测更敏感，并且两种用于VZV的新检测寡核苷酸在低水平病毒DNA时均能良好发挥作用。
发现修订后的HSV-PCR以及新的HSV和VZV寡核苷酸功能良好且更敏感，从而提高了该方法的可靠性。
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