Zaczynska Ewa, Duś Danuta, Paprocka Maria, Błach-Olszewska Zofia
Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland.
Folia Histochem Cytobiol. 2008;46(1):39-43. doi: 10.2478/v10042-008-0004-9.
Among reactions of innate immunity, resistance of human peripheral blood leukocytes (PBL) to viral infection seems important. The purpose of our study was to find, which of the subpopulations of PBL is the most responsible for the innate antiviral immunity of these cells. The innate immunity was measured by using the direct method of infection of leukocytes with vesicular stomatitis virus (VSV). The lack of VSV replication by infected leukocytes (0-1 log TCID50) was taken as an indicator for complete immunity; a low level of VSV (2-3 log) for partial immunity; and high VSV titer (more than 4 log) for no immunity. The resistance/innate immunity of whole PBL and subpopulations such as: adherent cells, fractions enriched in lymphocytes T, and lymphocytes B (separated on column with nylon wool), NK(+) and NK(-) (separated by microbeads activated cell sorting MACS) differ from each other. All fractions express higher resistance/innate immunity than the whole PBL. NK(+) cells were found the most resistant fraction of PBL to VSV infection. The results indicate that among the leukocytes in PBL the regulation mechanisms of innate immunity exist. The study on the mechanism of innate immunity regulation as well as the role of NK in innate immunity of PBL must be continued.
在先天性免疫反应中，人类外周血白细胞（PBL）对病毒感染的抵抗力似乎很重要。我们研究的目的是找出PBL的哪些亚群对这些细胞的先天性抗病毒免疫最为关键。通过使用水疱性口炎病毒（VSV）直接感染白细胞的方法来测量先天性免疫。被感染白细胞缺乏VSV复制（0 - 1 log TCID50）被视为完全免疫的指标；低水平的VSV（2 - 3 log）表示部分免疫；高VSV滴度（超过4 log）表示无免疫。整个PBL及其亚群如贴壁细胞、富含T淋巴细胞的组分、B淋巴细胞（通过尼龙毛柱分离）、NK(+)和NK(-)（通过微珠激活细胞分选MACS分离）的抵抗力/先天性免疫各不相同。所有组分都表现出比整个PBL更高的抵抗力/先天性免疫。发现NK(+)细胞是PBL中对VSV感染最具抵抗力的组分。结果表明，在PBL中的白细胞之间存在先天性免疫调节机制。对先天性免疫调节机制以及NK在PBL先天性免疫中的作用的研究必须继续进行。
