Brown C C, Malech H L, Jacobson R J, Shrimpton C F, Beverly P C, Segal A W, Gallin J I
Bacterial Diseases Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892.
Cell Immunol. 1991 Jan;132(1):102-14. doi: 10.1016/0008-8749(91)90010-9.
A mouse IgG1 monoclonal antibody ED12F8C10 (C10) binds a constant percentage of peripheral blood neutrophils in the same individual when studied over time, defining a distinct subset of neutrophils in all normal individuals studied to date. Bone marrow studies confirm that the heterogeneity is present to the same degree at all stages of neutrophil development from the myelocyte to the mature neutrophil. Neither in vivo nor in vitro activation of neutrophils explains or significantly alters the relative percentages of C10-positive and -negative neutrophils in the same individual. With both activation and exudation, however, expression of the C10-defined epitope increases in intensity in the C10 binding subpopulation. Studies of NBT reduction, phagocytosis, adherence, light scattering characteristics, and monoclonal antibody surface binding have failed to demonstrate physical or functional differences between the C10-defined populations. We examined C10 binding in patients with different defects of phagocyte function. In two patients with neutrophil-specific granule deficiency, less than 1% of the neutrophils were found to be C10 positive, while neutrophils from a patient with idiopathic leukemoid reaction and recurrent infections demonstrated greater than 99% C10 binding. Although the present study does not delineate the physiologic significance of C10 binding heterogeneity, it firmly supports the concept of neutrophil heterogeneity at the level of surface antigen expression.
小鼠IgG1单克隆抗体ED12F8C10（C10）在对同一个体进行长期研究时，会结合外周血中性粒细胞的恒定比例，这确定了在迄今所研究的所有正常个体中，中性粒细胞的一个独特亚群。骨髓研究证实，从髓细胞到成熟中性粒细胞的中性粒细胞发育的所有阶段，这种异质性都以相同程度存在。中性粒细胞在体内或体外的激活，均无法解释或显著改变同一个体中C10阳性和阴性中性粒细胞的相对比例。然而，在激活和渗出过程中，C10结合亚群中C10定义的表位的表达强度会增加。对硝基蓝四唑还原、吞噬作用、黏附、光散射特性以及单克隆抗体表面结合的研究，均未能证明C10定义的群体之间存在物理或功能差异。我们检查了具有不同吞噬细胞功能缺陷患者的C10结合情况。在两名患有中性粒细胞特异性颗粒缺乏症的患者中，发现不到1%的中性粒细胞为C10阳性，而一名患有特发性类白血病反应和反复感染的患者的中性粒细胞显示出超过99%的C10结合。虽然本研究没有阐明C10结合异质性的生理意义，但它有力地支持了中性粒细胞在表面抗原表达水平上存在异质性的概念。
