一氧化氮生物合成抑制剂N-硝基-L-精氨酸对绵羊的血流动力学和激素影响。

Haemodynamic and hormonal effects of N-nitro-L-arginine, an inhibitor of nitric oxide biosynthesis, in sheep.

Tresham J J, Dusting G J, Coghlan J P, Whitworth J A

Howard Florey Institute of Experimental Physiology and Medicine, Parkville, Victoria, Australia.

Clin Exp Pharmacol Physiol. 1991 May;18(5):327-30. doi: 10.1111/j.1440-1681.1991.tb01456.x.

Abstract

The haemodynamic and hormonal responses to N-nitro-L-arginine (NOLA), a potent inhibitor of nitric oxide biosynthesis in endothelial cells, were investigated in conscious sheep. 2. Mean arterial blood pressure (MAP), heart rate (HR) and cardiac output by thermodilution (CO) were measured in four oophrectomized ewes. Two other ewes were surgically implanted with aortic electromagnetic flow probes and an indwelling carotid arterial line for monitoring CO and MAP over 40 h. 3. After a control period, NOLA (10 mg/kg) was injected intravenously and MAP, HR and CO monitored and blood samples taken at intervals over the following 24 h. 4. NOLA increased blood pressure within minutes, from 76 +/- 4 to a maximum of 99 +/- 4 mmHg (P less than 0.001) at 6 h after injection. It remained elevated 24 h after injection. CO and HR fell but these falls were not sustained longer than 6 h. Calculated total peripheral resistance increased to a maximum of 2 h, but had returned to control levels 24 h after injection. There were no significant changes in plasma concentrations of renin, atrial natriuretic factor, vasopressin, noradrenaline or endothelin during the first hour. 5. NOLA may be a useful tool in understanding the role of the endothelium and nitric oxide in the control of blood pressure.

摘要

在清醒绵羊中研究了对N-硝基-L-精氨酸（NOLA，内皮细胞中一氧化氮生物合成的强效抑制剂）的血流动力学和激素反应。2. 在四只去卵巢母羊中测量了平均动脉血压（MAP）、心率（HR）和热稀释法测定的心输出量（CO）。另外两只母羊通过手术植入主动脉电磁流量探头和留置颈动脉导管，用于在40小时内监测CO和MAP。3. 在对照期后，静脉注射NOLA（10mg/kg），并在接下来的24小时内间隔监测MAP、HR和CO，同时采集血样。4. NOLA在数分钟内使血压升高，注射后6小时血压从76±4mmHg升至最高99±4mmHg（P<0.001）。注射后24小时血压仍保持升高。CO和HR下降，但这些下降持续时间不超过6小时。计算得出的总外周阻力在2小时时升至最高，但注射后24小时已恢复至对照水平。在最初的一小时内，血浆肾素、心房利钠因子、血管加压素、去甲肾上腺素或内皮素的浓度没有显著变化。5. NOLA可能是理解内皮和一氧化氮在血压控制中作用的有用工具。