Samulin Johanna, Lien Sigbjørn, Grindflek Eli, Berget Ingunn, Ruyter Bente, Sundvold Hilde
Department of Animal and Aquacultural Sciences, Norwegian University of Life Sciences, PO Box 5003, N-1432 Aas, Norway.
Cell Biol Int. 2008 May;32(5):525-31. doi: 10.1016/j.cellbi.2008.01.001. Epub 2008 Jan 25.
Recently a role of adipose tissue as an endocrine organ secreting factors involved in the regulation of whole-body energy homeostasis has emerged. Preadipocytes in different fat depots have distinct adipogenic potential and the metabolic activity differs between mature adipocytes of different depot origins. Here we describe the proliferation and differentiation of stromal-vascular cells derived from subcutaneous and visceral fat depots of adult pigs. We demonstrate that subcutaneous porcine preadipocytes proliferate more actively and that individual subcutaneous adipocytes have a more rapid accumulation of triacylglycerols than visceral cells. During differentiation, subcutaneous and visceral preadipocytes showed similar gene expression patterns with increased expression of adiponectin (APM1), adipocyte-specific fatty acid binding protein (FABP4), catalase (CAT), and peroxisome proliferator-activated receptor gamma 2 (PPARG2). Furthermore, initial data showing depot-originated effects on the expression of CAT, carnitine palmitoyl transferase 1B (CPT1B) and FABP4 suggest possible depot specific differences in the function and metabolism of mature porcine adipocytes.
最近，脂肪组织作为一个分泌参与全身能量稳态调节因子的内分泌器官的作用已逐渐显现。不同脂肪库中的前脂肪细胞具有不同的成脂潜能，且不同库来源的成熟脂肪细胞之间的代谢活性也有所不同。在此，我们描述了源自成年猪皮下和内脏脂肪库的基质血管细胞的增殖和分化。我们证明，猪皮下前脂肪细胞增殖更活跃，且单个皮下脂肪细胞比内脏细胞有更快的三酰甘油积累。在分化过程中，皮下和内脏前脂肪细胞表现出相似的基因表达模式，脂联素（APM1）、脂肪细胞特异性脂肪酸结合蛋白（FABP4）、过氧化氢酶（CAT）和过氧化物酶体增殖物激活受体γ2（PPARG2）的表达增加。此外，初步数据显示库来源对CAT、肉碱棕榈酰转移酶1B（CPT1B）和FABP4表达的影响，提示成熟猪脂肪细胞在功能和代谢方面可能存在库特异性差异。
