Ameri Hossein, Kim June-Gone, Ratanapakorn Tanapat, Chader Gerald J, Humayun Mark S
Doheny Retina Institute, Doheny Eye Institute, Department of Ophthalmology, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA.
Retina. 2008 Feb;28(2):350-5. doi: 10.1097/IAE.0b013e318140580c.
To evaluate the effectiveness of intravitreal or subretinal injection of tPA in the treatment of experimentally created retinal vein occlusion (RVO) in rabbits.
Fifteen rabbits were included in this study. RVO was created in all using an argon green laser following intravenous injection of rose Bengal. Follow-up examinations included color fundus photography, fundus fluorescein angiography, and optical coherence tomography. Following examinations at day 2, animals were divided into three groups: six rabbits were kept as control, four rabbits received subretinal injection of tPA, and five rabbits received intravitreal tPA.
Of 14 eyes that had capillary drop out at day 2 following creation of RVO, 12 remained the same at week 3. Only one eye in the control group and one eye in the subretinal group developed complete reperfusion of capillaries. Although there was more revascularization in all groups at week 3, there was not a significant difference in the amount of revascularization between the control group and those animals receiving subretinal or intravitreal tPA.
Intravitreal or subretinal injection of tPA 2 days following vein occlusion did not improve reperfusion of retinal vessels in experimentally created RVO in rabbits when compared to control group.
评估玻璃体内或视网膜下注射组织型纤溶酶原激活剂（tPA）治疗实验性兔视网膜静脉阻塞（RVO）的有效性。
本研究纳入15只兔。所有兔静脉注射孟加拉玫瑰红后，用氩绿激光造成RVO。随访检查包括彩色眼底照相、眼底荧光血管造影和光学相干断层扫描。在第2天检查后，将动物分为三组：6只兔作为对照组，4只兔接受视网膜下注射tPA，5只兔接受玻璃体内注射tPA。
在造成RVO后第2天出现毛细血管闭塞的14只眼中，12只在第3周时情况保持不变。对照组和视网膜下注射组各有1只眼出现毛细血管完全再灌注。虽然在第3周时所有组的血管再生情况都有所增加，但对照组与接受视网膜下或玻璃体内注射tPA的动物之间，血管再生量没有显著差异。
与对照组相比，静脉阻塞2天后玻璃体内或视网膜下注射tPA并不能改善实验性兔RVO中视网膜血管的再灌注。
