Lutton B V, Callard I P
Transplantation Biology Research Center, Massachusetts General Hospital, MGH East, Building 149-9019 13th Street, Charlestown, Massachusetts 02129, USA.
J Morphol. 2008 May;269(5):620-9. doi: 10.1002/jmor.10614.
In elasmobranchs, a unique association exists between an immune tissue, the epigonal organ (EO), and the gonads. In this study, the histological and vascular relationships of the EO and ovarian follicles of the little skate, Leucoraja erinacea, were assessed. Perfusions of Evans blue dye and Batson's monomer showed a shared vascular pathway from the gonadal artery into the epigonal-ovary complex, with blood first entering the EO and then perfusing the ovarian follicles. Histological studies demonstrated direct cellular contact between epigonal leukocytes and the follicle wall (FW), as well as the presence of leukocytes between the steroidogenic theca and granulosa cells. In vitro analyses demonstrated that epigonal cells co-cultured with FW cells cause a dose-dependent inhibition of estrogen (E2) and testosterone (T) production. In contrast, conditioned media from epigonal leukocytes, stimulated or unstimulated with lipopolysaccharide (10 microg/ml), increase the production of E2 and T from FW cells of the ovaries. These studies provide a basis for further investigations of leukocyte secreted factors and cell contact modulation of follicular steroid production.
在软骨鱼类中，一种独特的关联存在于一种免疫组织——性腺旁器官（EO）和性腺之间。在本研究中，对小鳐（Leucoraja erinacea）的性腺旁器官和卵巢卵泡的组织学及血管关系进行了评估。伊文思蓝染料和巴特森单体灌注显示，从性腺动脉到性腺旁 - 卵巢复合体存在一条共享的血管通路，血液首先进入性腺旁器官，然后灌注卵巢卵泡。组织学研究表明，性腺旁白细胞与卵泡壁（FW）之间存在直接细胞接触，并且在类固醇生成的卵泡膜细胞和颗粒细胞之间也存在白细胞。体外分析表明，与FW细胞共培养的性腺旁细胞会导致雌激素（E2）和睾酮（T）生成的剂量依赖性抑制。相反，用脂多糖（10微克/毫升）刺激或未刺激的性腺旁白细胞的条件培养基会增加卵巢FW细胞中E2和T的生成。这些研究为进一步研究白细胞分泌因子以及卵泡类固醇生成的细胞接触调节提供了基础。
