Holmes Peta, Goffard Nicolas, Weiller Georg F, Rolfe Barry G, Imin Nijat
ARC Centre of Excellence for Integrative Legume Research, Genomic Interactions Group, Research School of Biological Sciences, Australian National University, Canberra ACT 2601, Australia.
BMC Plant Biol. 2008 Feb 27;8:21. doi: 10.1186/1471-2229-8-21.
The root apical meristem of crop and model legume Medicago truncatula is a significantly different stem cell system to that of the widely studied model plant species Arabidopsis thaliana. In this study we used the Affymetrix Medicago GeneChip(R) to compare the transcriptomes of meristem and non-meristematic root to identify root meristem specific candidate genes.
Using mRNA from root meristem and non-meristem we were able to identify 324 and 363 transcripts differentially expressed from the two regions. With bioinformatics tools developed to functionally annotate the Medicago genome array we could identify significant changes in metabolism, signalling and the differentially expression of 55 transcription factors in meristematic and non-meristematic roots.
This is the first comprehensive analysis of M. truncatula root meristem cells using this genome array. This data will facilitate the mapping of regulatory and metabolic networks involved in the open root meristem of M. truncatula and provides candidates for functional analysis.
作物和模式豆科植物蒺藜苜蓿的根尖分生组织是一种与广泛研究的模式植物拟南芥显著不同的干细胞系统。在本研究中，我们使用Affymetrix Medicago GeneChip(R)比较分生组织和非分生组织根的转录组，以鉴定根分生组织特异性候选基因。
利用根分生组织和非分生组织的mRNA，我们能够鉴定出在这两个区域差异表达的324个和363个转录本。通过开发的用于对蒺藜苜蓿基因组阵列进行功能注释的生物信息学工具，我们可以识别出分生组织根和非分生组织根中代谢、信号传导的显著变化以及55个转录因子的差异表达。
这是首次使用该基因组阵列对蒺藜苜蓿根分生组织细胞进行全面分析。这些数据将有助于绘制蒺藜苜蓿开放根分生组织中涉及的调控和代谢网络，并为功能分析提供候选基因。
