Zhang Huizhen, Ma Aiguo, Liang Hui, Sun Yongye
Institute of Nutrition, Medical College of Qingdao University, Qingdao 266021, China.
Wei Sheng Yan Jiu. 2007 Nov;36(6):748-50.
To estabish a high performance liquid chromatographic (HPLC) method for the determination of dehydroaetic acid in food determinated.
Separation was carried out on a column of C18 (51 microm, 250 x 4.60mm) at the temperature of 25 degrees C with methanol/0.02mol/L ammonium acetate (5:95, V/V) as mobile phase, and elution flow rate was 1.0ml/min. The detection wavelength was 306nm and the injection volume was 10 microl, quantitation was used by external standard method.
The results showed a good linear relationship between the peak area and the concentration (r = 0.9997), the recoveries for Dehydroaetic acid products were 96.5% - 99.5%, and the relative standard deviation was 1.09% - 5.85% for different kinds of food.
The results showed that the method may be simple, rapid, accurate and applicable for the determination of dehydroaetic acid in food.
建立一种高效液相色谱法（HPLC）测定食品中脱氢乙酸的含量。
采用C18柱（5μm，250×4.60mm），在25℃下，以甲醇/0.02mol/L醋酸铵（5:95，V/V）为流动相，洗脱流速为1.0ml/min。检测波长为306nm，进样量为10μl，采用外标法进行定量。
结果表明峰面积与浓度之间具有良好的线性关系（r = 0.9997），脱氢乙酸产品的回收率为96.5% - 99.5%，不同种类食品的相对标准偏差为1.09% - 5.85%。
结果表明该方法简便、快速、准确，适用于食品中脱氢乙酸的测定。
