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[一种用于病原体检测和鉴定的快速、多重新技术——xMAP 液相芯片]

[A rapid, multiplexed new technology xMAP liquid chip for detection and identification of pathogens].

作者信息

Hu Rui, Wang Jinglin

机构信息

College of Animal Science and Technology, Northwest A & F University, Yangling 712100, China.

出版信息

Wei Sheng Yan Jiu. 2007 Nov;36(6):759-62.

Abstract

xMAP liquid chip is a new. biochip technology developed by the Luminex corporation in recent years. This chip technology based on microsphere is capable of simultaneously detecting up to 100 different analytes in a single reaction vessel. In comparition with the solid chip or membrane chip, it possesses the features of multi-analytes, high sensitivity (0.01 pg), good reproducibility (CV < 5%), wide channel (0.2 - 32000 pg/ml), and so on. For these advantages, the xMAP liquid chip technology has been used in many fields, especially in the analysis and identification of nucleic acid, protein or other biological molecules.

摘要

xMAP 液态芯片是Luminex公司近年来开发的一项新型生物芯片技术。这种基于微球的芯片技术能够在单个反应容器中同时检测多达100种不同的分析物。与固体芯片或膜芯片相比,它具有多分析物、高灵敏度(0.01皮克)、良好的重现性(变异系数<5%)、宽检测范围(0.2 - 32000皮克/毫升)等特点。由于这些优势,xMAP液态芯片技术已在许多领域得到应用,尤其是在核酸、蛋白质或其他生物分子的分析和鉴定方面。

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