Kim Jin-Hyoung, Raisuddin Sheikh, Ki Jang-Seu, Lee Jae-Seong, Han Kyung-Nam
Department of Marine Science, College of Natural Sciences, Inha University, Incheon 402-751, South Korea.
Mar Pollut Bull. 2008;57(6-12):433-40. doi: 10.1016/j.marpolbul.2008.01.005. Epub 2008 Mar 4.
In recent years, there has been a decline in the wild populations of river pufferfish, Takifugu obscurus. Besides overexploitation for commercial purposes, environmental pollution is believed to have contributed to its decline. However, almost no information exists about genes involved in metabolism of xenobiotics by this species. Nevertheless, there is interest in fugu fishes, since they possess the smallest genome among vertebrates. We cloned and characterized the full-length cDNA sequence of a cytochrome P450 1A (CYP1A) gene from T. obscurus. Phylogenic relationship of T. obscurus CYP1A was also compared to other fish species. The tissue distribution and time-dependant induction of CYP1A mRNA were studied by real-time PCR in T. obscurus exposed to an aryl hydrocarbon receptor (Ahr) agonist, beta-naphthoflavone (BNF). The greatest basal expression in livers of control as well as BNF-treated individuals. However, brain, gill, gonad, intestine, and kidney also expressed CYP1A. Muscles expressed the least CYP1A. The results of the time-course study revealed induction in brain and gills after 6h and at 12h in most tissues. Except for gills, all other organs retained induced expression of CYP1A mRNA up to 96h.
近年来，野生暗纹东方鲀的种群数量有所下降。除了因商业目的的过度捕捞外，环境污染也被认为是导致其数量下降的原因之一。然而，关于该物种参与外源性物质代谢的基因，几乎没有相关信息。尽管如此，河豚鱼类仍受到关注，因为它们拥有脊椎动物中最小的基因组。我们克隆并鉴定了暗纹东方鲀细胞色素P450 1A（CYP1A）基因的全长cDNA序列。还将暗纹东方鲀CYP1A的系统发育关系与其他鱼类进行了比较。通过实时PCR研究了暴露于芳烃受体（Ahr）激动剂β-萘黄酮（BNF）的暗纹东方鲀中CYP1A mRNA的组织分布和时间依赖性诱导情况。对照组以及BNF处理组个体的肝脏中基础表达量最高。然而，脑、鳃、性腺、肠道和肾脏也表达CYP1A。肌肉中CYP1A的表达量最少。时间进程研究结果显示，大多数组织在6小时和12小时后，脑和鳃中出现诱导现象。除鳃外，所有其他器官中CYP1A mRNA的诱导表达可持续至96小时。
