用于检测双链DNA的菁染料。

Cyanine dyes for the detection of double stranded DNA.

作者信息

Hilal H, Taylor J A

机构信息

School of Materials, Faculty of Engineering and Physical Sciences, University of Manchester, M60 1QD, United Kingdom.

出版信息

J Biochem Biophys Methods. 2008 Apr 24;70(6):1104-8. doi: 10.1016/j.jprot.2007.12.008. Epub 2008 Jan 7.

DOI:10.1016/j.jprot.2007.12.008

PMID:18304645

Abstract

Twenty three novel cyanine dyes have been applied as fluorescent stains for the detection of nucleic acids in agarose gel electrophoresis. Significant fluorescence enhancement of these dyes in the presence of double stranded DNA was observed. Five dyes offered superior sensitivity in the detection and quantification of DNA, over Ethidium Bromide, the most commonly used nucleic acid stain.

摘要

23种新型花青染料已被用作荧光染色剂，用于在琼脂糖凝胶电泳中检测核酸。在双链DNA存在的情况下，观察到这些染料有显著的荧光增强。与最常用的核酸染色剂溴化乙锭相比，有5种染料在DNA检测和定量方面具有更高的灵敏度。