微粒体钙转运酶抑制剂毒胡萝卜素和2,5-二（叔丁基）-1,4-苯二酚对细胞钙通量影响的比较。
Comparison between the effects of the microsomal Ca(2+)-translocase inhibitors thapsigargin and 2,5-di-(t-butyl)-1,4-benzohydroquinone on cellular calcium fluxes.
作者信息
Llopis J, Chow S B, Kass G E, Gahm A, Orrenius S
机构信息
Department of Toxicology, Karolinska Institutet, Stockholm, Sweden.
出版信息
Biochem J. 1991 Jul 15;277 ( Pt 2)(Pt 2):553-6. doi: 10.1042/bj2770553.
Abstract
The effects of two inhibitors of the microsomal Ca(2+)-ATPase, thapsigargin and 2,5-di-(t-butyl)-1,4-benzohydroquinone, were compared in hepatocytes and in a T-cell line (JURKAT). Both compounds mobilized the same intracellular Ca2+ pool, which contained the Ins(1,4,5)P3-sensitive store, in hepatocytes and in JURKAT cells. The mobilization of the internal Ca2+ store with either compound activated Mn2+ entry in JURKAT cells, but not in hepatocytes. This suggests different properties of the bivalent-cation entry pathway between these cell types.
摘要
在肝细胞和一种T细胞系（JURKAT）中比较了两种微粒体Ca(2+)-ATP酶抑制剂毒胡萝卜素和2,5-二（叔丁基）-1,4-苯二酚的作用。在肝细胞和JURKAT细胞中，这两种化合物均动员了同一个细胞内Ca2+池，该池含有对肌醇-1,4,5-三磷酸（Ins(1,4,5)P3）敏感的储存库。两种化合物中的任何一种对细胞内Ca2+储存库的动员均激活了JURKAT细胞中的Mn2+内流，但未激活肝细胞中的Mn2+内流。这表明这些细胞类型之间二价阳离子内流途径具有不同特性。
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