Guggiari Matteo, Peck Robert
Department of Zoology and Animal Biology, University of Geneva, 1211 Geneva 4, Switzerland.
Eur J Protistol. 2008 Aug;44(3):168-80. doi: 10.1016/j.ejop.2007.11.004. Epub 2008 Mar 4.
A strain of Cyclidium isolated from a sewage treatment plant in Geneva, Switzerland, was cloned and cultured under laboratory conditions before being characterized cytologically and molecularly. Information about the classical morphology and also about new molecular sequences has been obtained permitting its identification as Cyclidium glaucoma. The molecular description includes the 18S rRNA sequence, and for the first time for C. glaucoma the ITS-1, 5.8S, ITS-2 sequence, and the mitochondrial Cytochrome oxidase C subunit 1 sequence. Three divergence groups were identified for C. glaucoma using a short rDNA sequence for evolutionary distance analysis. These groups correspond to the three ribotype lineages previously described for C. glaucoma. We discuss the possibility that C. glaucoma is a species complex containing three cryptic species, the problems of species definition in the protozoa and the choice of a barcoding molecule for the ciliates.
从瑞士日内瓦一家污水处理厂分离出的一株睫滴虫进行了克隆，并在实验室条件下培养，随后进行了细胞学和分子学特征分析。已获得有关其经典形态以及新分子序列的信息，从而得以将其鉴定为眼睫滴虫。分子描述包括18S rRNA序列，并且首次获得了眼睫滴虫的ITS-1、5.8S、ITS-2序列以及线粒体细胞色素氧化酶C亚基1序列。利用短rDNA序列进行进化距离分析，确定了眼睫滴虫的三个分化组。这些组对应于先前描述的眼睫滴虫的三个核糖体谱系。我们讨论了眼睫滴虫是一个包含三个隐性物种的复合物种的可能性、原生动物物种定义的问题以及纤毛虫条形码分子的选择。
