Goddard Matthew, Zheng Yiwen, Darlington Cynthia L, Smith Paul F
Department of Pharmacology and Toxicology, School of Medical Sciences, University of Otago, Dunedin, New Zealand.
Hippocampus. 2008;18(5):440-4. doi: 10.1002/hipo.20416.
Several studies have reported that bilateral vestibular deafferentation (BVD) results in the disruption of place cell function and theta activity in the hippocampus. Recent magnetic resonance imaging (MRI) studies in humans demonstrated that bilateral but not unilateral vestibular loss is associated with a bilateral atrophy of the hippocampus. In this study we investigated whether BVD in rats resulted in changes in the expression of four proteins related to neuronal plasticity, synaptophysin, SNAP-25, drebrin and neurofilament-L, in the hippocampal subregions (CA1, CA2/3, the DG) and the entorhinal (EC), perirhinal (PRC) and frontal cortices (FC), using western blotting. At 6 months following BVD, there were no significant differences in the expression of synaptophysin in any region. There were also no significant differences in SNAP-25 expression in CA1, CA2/3, EC, PRC, or the FC; however, there was a significant increase in SNAP-25 expression in the DG compared to sham controls. Drebrin A and E expression was significantly reduced in the EC and drebrin A was significantly reduced in the FC of BVD animals. NF-L expression was not significantly different in CA1, CA2/3, DG, EC, or the PRC. However, its expression was significantly reduced in the FC of BVD animals. These data suggest that circumscribed neurochemical changes in SNAP-25, drebrin and NF-L expression occur in the DG, EC, and the FC over 6 months following BVD.
多项研究报告称，双侧前庭去传入（BVD）会导致海马体中位置细胞功能和θ活动的破坏。最近针对人类的磁共振成像（MRI）研究表明，双侧而非单侧前庭丧失与海马体的双侧萎缩有关。在本研究中，我们使用蛋白质印迹法，研究了大鼠的BVD是否会导致海马亚区（CA1、CA2/3、齿状回（DG））以及内嗅皮质（EC）、梨状皮质（PRC）和额叶皮质（FC）中与神经元可塑性相关的四种蛋白质，即突触素、SNAP-25、脑桥蛋白和神经丝轻链（NF-L）的表达发生变化。BVD术后6个月时，任何区域的突触素表达均无显著差异。CA1、CA2/3、EC、PRC或FC中的SNAP-25表达也无显著差异；然而，与假手术对照组相比，DG中的SNAP-25表达显著增加。BVD动物的EC中脑桥蛋白A和E的表达显著降低，FC中脑桥蛋白A的表达显著降低。CA1、CA2/3、DG、EC或PRC中的NF-L表达无显著差异。然而，BVD动物FC中的NF-L表达显著降低。这些数据表明，BVD术后6个月内，DG、EC和FC中SNAP-25、脑桥蛋白和NF-L的表达发生了局限性神经化学变化。
