Meng Tao, Xu Duan-fu, Zhao Ying, Wang Du-jin, Xu Yi-zhuang, Qi Jian, Wu Jin-guang
State Key Laboratory of Polymer Physics and Chemistry, Joint Laboratory of Polymer Science and Materials, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China.
Guang Pu Xue Yu Guang Pu Fen Xi. 2007 Oct;27(10):2069-72.
A new fluorescent staining method to distinguish normal and malignant rectum tissues using terbium(III)-Ciprofloxacin complex was investigated. The terbium (III) complex exhibits strong fluorescence and the staining process with the complex is very fast. It needs 3-5 minutes. The results showed that the fluorescence microscope images were similar to the images stained using traditional hematoxylin and eosin (H&E) methodology. The fluorescence spectra and FTIR spectra of normal and malignant rectum tissues were collected and the detection criterions for the malignant rectum tissues were the same as the staining results. In summary, the fluorescence staining may be developed as a simple and convenient method.
研究了一种使用铽(III)-环丙沙星复合物区分正常和恶性直肠组织的新型荧光染色方法。铽(III)复合物表现出强荧光,且用该复合物进行染色的过程非常快,只需3-5分钟。结果表明,荧光显微镜图像与使用传统苏木精和伊红(H&E)方法染色的图像相似。收集了正常和恶性直肠组织的荧光光谱和傅里叶变换红外光谱(FTIR),恶性直肠组织的检测标准与染色结果一致。总之,荧光染色可能发展成为一种简单便捷的方法。