Gao Xiu-xiang, He Wen-yi, Yao Hong-wei, Du Jun-kai, Zhao Mei-xian, Qi Jian, Li Hui-zhen, Xu Yi-zhuang, Wu Jin-guang
College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.
Guang Pu Xue Yu Guang Pu Fen Xi. 2008 Sep;28(9):2201-6.
In the present paper, NMR spectroscopy, an effective tool to detect the variation in molecular structure and changes in chemical composition of metabolites in tissues, was used to study the differences between malignant and normal tissues from rectum. 1H spectra of four malignant rectum tissue samples and two normal control tissues were investigated by using a 500M NMR high-resolution magic angle spinning magnetic resonance spectrometers (HR-MAS NMR). The results indicate that the 1H HR-MAS spectra of rectum cancer tissues are significantly different from those of the normal controls and most differences are presents in the form of variation in the relative intensities of the characteristic peak of various metabolites. In order to characterize the variation in the relative intensities in a quantitative manner, the intensity of the methyl peak of fatty acid at 0.88 was utilized as inner standard. Systematic differences between NMR spectra of malignant tissue and normal controls are as follows: (1) The concentration of amino acid increases significantly in malignant tissues, since the relative intensities of characteristic peaks of amino acid including valine, isoleucine, leucine, lysine, glutamate, glutamine, and aspartate are stronger in the NMR spectra of the malignant tissues. This phenomenon may reflect the fact that the activity of protein synthesis is enhanced in cancerous tissues. (2) The intensities of the characteristic peaks of lactic acid in malignant tissues are higher than those from normal controls. This may be related to the nature of anaerobic metabolism activity in malignant tissues. (3) The level of choline and its derivatives, taurine and creatine, increases significantly in malignant tissues, suggesting that the metabolic activity of malignant tissues changes. (4) In the spectral region between 4.5 and 10, observable changes occur on the peaks for unsaturated fatty acid and nuclear acids. Therefore, the above spectral variations in high resolution magic angle spinning NMR spectroscopy may be utilized as a potential tool to diagnose rectum cancer.
在本论文中，核磁共振波谱法作为一种检测组织中代谢物分子结构变化和化学成分改变的有效工具，被用于研究直肠恶性组织与正常组织之间的差异。使用一台500M核磁共振高分辨魔角旋转磁共振光谱仪（HR-MAS NMR）对四个直肠恶性组织样本和两个正常对照组织的1H谱进行了研究。结果表明，直肠癌组织的1H HR-MAS谱与正常对照组织的谱有显著差异，且大多数差异以各种代谢物特征峰相对强度的变化形式呈现。为了定量表征相对强度的变化，将0.88处脂肪酸甲基峰的强度用作内标。恶性组织与正常对照的核磁共振谱之间的系统差异如下：（1）恶性组织中氨基酸浓度显著增加，因为在恶性组织的核磁共振谱中，包括缬氨酸、异亮氨酸、亮氨酸、赖氨酸、谷氨酸、谷氨酰胺和天冬氨酸在内的氨基酸特征峰相对强度更强。这种现象可能反映了癌组织中蛋白质合成活性增强这一事实。（2）恶性组织中乳酸特征峰的强度高于正常对照组织。这可能与恶性组织中无氧代谢活性的性质有关。（3）恶性组织中胆碱及其衍生物、牛磺酸和肌酸的水平显著升高，表明恶性组织的代谢活性发生了变化。（4）在4.5至10之间的光谱区域，不饱和脂肪酸和核酸的峰出现了可观察到的变化。因此，上述高分辨魔角旋转核磁共振光谱中的光谱变化可作为诊断直肠癌的潜在工具。
