Yukelson L Y, Tans G, Thomassen M C, Hemker H C, Rosing J
Institute of Biochemistry of the Uzbek Academy of Sciences, Tashkent, U.S.S.R.
Toxicon. 1991;29(4-5):491-502. doi: 10.1016/0041-0101(91)90023-k.
The venoms from central Asian snakes (Echis carinatus, Echis multisquamatus, Vipera ursini, Vipera lebetina, Agkistrodon halys halys and Naja naja oxiana) contain several enzymes with amidolytic- and procoagulant activity. We have characterized the activities and the mol. wts of the venom enzymes that are able to convert a number of commercially available chromogenic substrates for activated coagulation factors. The chromogenic substrate cleavage patterns obtained for the crude venoms may be helpful tools in the further identification of venom fractions and venom enzymes with procoagulant activity. The crude venoms were also tested for their ability to clot fibrinogen, to lyse fibrin polymers and to activate the coagulation factors prothrombin, factor X and factor V. The products of venom-catalyzed coagulation factor activation were structurally characterized by SDS gel electrophoresis and were compared with activated coagulation factors that are generated under physiological conditions.
中亚蛇类（锯鳞蝰、多鳞锯鳞蝰、乌尔米蝰蛇、草原蝰、白眉蝮和中亚眼镜蛇）的毒液含有多种具有酰胺分解活性和促凝血活性的酶。我们已经对能够转化多种市售用于活化凝血因子的显色底物的毒液酶的活性和分子量进行了表征。从粗毒液获得的显色底物切割模式可能有助于进一步鉴定具有促凝血活性的毒液组分和毒液酶。还测试了粗毒液使纤维蛋白原凝固、溶解纤维蛋白聚合物以及激活凝血因子凝血酶原、因子X和因子V的能力。通过SDS凝胶电泳对毒液催化的凝血因子活化产物进行结构表征，并与在生理条件下产生的活化凝血因子进行比较。
