蛇毒中神经生长因子的单克隆抗体免疫亲和层析法
Monoclonal antibody immunoaffinity chromatography of the nerve growth factor from snake venoms.
作者信息
Siigur J, Arumäe U, Neuman T, Siigur E, Saarma M
出版信息
Comp Biochem Physiol B. 1987;87(2):329-34. doi: 10.1016/0305-0491(87)90147-7.
PMID:3621902
Abstract
- Pure monoclonal antibodies to Vipera lebetina venom nerve growth factor have been isolated by affinity chromatography using CNBr-agarose bound antigen. 2. Nerve growth factors from ten snake venoms (Vipera lebetina, Vipera russellii, Vipera berus berus, Vipera ursini, Echis carinatus, Agkistrodon halys, Bungarus caeruleus, Naja naja oxiana, Naja naja, Naja naja atra) were purified using monoclonal antibodies against NGF linked to BrCN-activated agarose.
摘要
- 利用结合了抗原的溴化氰活化琼脂糖通过亲和层析法分离出了针对草原蝰蛇毒神经生长因子的纯单克隆抗体。2. 使用与溴化氰活化琼脂糖相连的抗神经生长因子单克隆抗体，对来自十种蛇毒（草原蝰蛇、罗素蝰蛇、贝氏蝰蛇、乌氏蝰蛇、锯鳞蝰蛇、白眉蝮蛇、蓝环蛇、中亚眼镜蛇、眼镜蛇、舟山眼镜蛇）的神经生长因子进行了纯化。
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