Sugimoto K, Sakurai N, Shirasawa H, Kaneko M, Fujise Y, Shibata K, Komori Y, Nikai T, Sugihara H, Fukuda Y
Department of Hygiene, Hamamatsu University School of Medicine, Japan.
J Vet Med Sci. 1991 Apr;53(2):255-62. doi: 10.1292/jvms.53.255.
The inhibitory effects of a prescription of herbal medicine, tentatively named P-3, were studied pathologically in an experimental model of the glomerular lesion induced by purified snake Agkistrodon acutus venom proteinase (Ac1-P) in mice. Ac1-P was intravenously inoculated at a single LD50 dose. In the treated group, mice were intraperitoneally injected with an extract of P-3 at a designated time once every two days from 2 days before to 1 week after Ac1-P inoculation. The control group mice were injected with saline instead of P-3. In the control group, the main pathologic changes within 48 hrs after inoculation were pulmonary and gastrointestinal tract hemorrhage and renal petechiae with hematuria. The kidney microscopically showed cystic transformation of the glomerular capillary tufts. followed by occlusive thrombosis. One week after inoculation, the glomerular lesions were mostly replaced by proliferative or proliferative-sclerosing changes with occasional crescent formation. Early signs of tubular atrophy accompanying the glomerular changes were observed. In the P-3 treated mice surviving 48 hrs and 1 week, the changes observed in the controls were markedly inhibited, although P-3 treated mice dying earlier than 30 hrs exhibited hemorrhagic changes similar to controls. This indicated that the herbal medicine had efficacy against the tissue injuries induced by Ac1-P as a proteolytic enzyme.
在小鼠纯化的尖吻蝮蛇毒蛋白酶（Ac1-P）诱导的肾小球病变实验模型中，对一种暂名为P-3的草药方剂的抑制作用进行了病理学研究。以单一半数致死剂量静脉接种Ac1-P。在治疗组中，从接种Ac1-P前2天至接种后1周，每隔两天在指定时间给小鼠腹腔注射一次P-3提取物。对照组小鼠注射生理盐水而非P-3。在对照组中，接种后48小时内的主要病理变化为肺和胃肠道出血以及肾瘀点伴血尿。肾脏显微镜检查显示肾小球毛细血管丛呈囊性变，随后出现闭塞性血栓形成。接种后1周，肾小球病变大多被增殖性或增殖性硬化性改变取代，偶尔有新月体形成。观察到伴随肾小球变化的肾小管萎缩早期迹象。在存活48小时和1周的P-3治疗小鼠中，尽管早于30小时死亡的P-3治疗小鼠表现出与对照组相似的出血性变化，但对照组中观察到的变化明显受到抑制。这表明该草药方剂对Ac1-P作为蛋白水解酶诱导的组织损伤具有疗效。
