[Cloning and bioinformatics analysis of P450 cDNA in Artemisia annua].
作者信息
Kong Jian-Qiang, Wang Wei, Zheng Xiao-Dong, Zhu Ping, Cheng Ke-Di
机构信息
Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2007 Nov;32(21):2227-31.
OBJECTIVE
Cloning and bioinformatics analysis of P450 cDNA in Artemisia annua.
METHOD
A P450 cDNA gene was cloned from A. annua by RT-PCR. The bioinformatics analysis of the P450 gene was performed.
RESULT
The complete ORF of this P450 cDNA is 1 464 bp and encodes 488 aa. The sequence was reported to GenBank and coded as DQ667171. Bioinformatics analysis of the P450 cDNA showed it encoded an A-type P450 protein with 54. 992 kDa, it's isoelectric point was 8.83 and the possibility of export to mitochondria was 0.893 2.
CONCLUSION
The comparable analysis of the P450 with CYP71AV1 revealed that the two proteins probably performed the same function because of the similar character.
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