Xu Li, Ding Zhi-Shan, Zhou Yun-Kai, Tao Xue-Fen
Zhejiang Chinese Medical Univevsity, Hangzhou 310053, China.
Zhong Yao Cai. 2009 Jun;32(6):852-6.
To obtain the full-length cDNA sequence of Secoisolariciresinol Dehydrogenase gene from Dysosma versipellis by RACE PCR,then investigate the character of Secoisolariciresinol Dehydrogenase gene.
The full-length cDNA sequence of Secoisolariciresinol Dehydrogenase gene was obtained by 3'-RACE and 5'-RACE from Dysosma versipellis.
We first reported the full cDNA sequences of Secoisolariciresinol Dehydrogenase in Dysosma versipellis. The acquired gene was 991bp in full length, including 5' untranslated region of 42bp, 3' untranslated region of 112bp with Poly (A). The open reading frame (ORF) encoding 278 amino acid with molecular weight 29253.3 Daltons and isolectric point 6.328. The gene accession nucleotide sequence number in GeneBank was EU573789. Semi-quantitative RT-PCR analysis revealed that the Secoisolariciresinol Dehydrogenase gene was highly expressed in stem. Alignment of the amino acid sequence of Secoisolariciresinol Dehydrogenase indicated there may be some significant amino acid sequence difference among different species.
Obtain the full-length cDNA sequence of Secoisolariciresinol Dehydrogenase gene from Dysosma versipellis.
通过RACE PCR技术获得八角莲中开环异落叶松脂素脱氢酶基因的全长cDNA序列，并对该基因的特性进行研究。
采用3'-RACE和5'-RACE技术从八角莲中获得开环异落叶松脂素脱氢酶基因的全长cDNA序列。
首次报道了八角莲中开环异落叶松脂素脱氢酶的cDNA全序列。获得的基因全长991bp，其中5'非翻译区为42bp，3'非翻译区为112bp并带有Poly (A)。开放阅读框（ORF）编码278个氨基酸，分子量为29253.3道尔顿，等电点为6.328。该基因在GenBank中的登录核苷酸序列编号为EU573789。半定量RT-PCR分析表明，开环异落叶松脂素脱氢酶基因在茎中高表达。开环异落叶松脂素脱氢酶氨基酸序列比对表明，不同物种之间可能存在一些显著的氨基酸序列差异。
获得了八角莲中开环异落叶松脂素脱氢酶基因的全长cDNA序列。
